| Assay Method Information | |
| | Fluorometric TLC Kinase Assay |
| Description: | The IC50 values of the inhibitors corresponding to the concentration of the inhibitor decreasing the enzyme activity 50% were measured using fluorescent-labeled substrate. The phosphorylation reaction was initiated by the addition of ATP. At fixed time points, the reaction was analyzed by normal phase TLC. The visualization and quantification of the fluorescent spots were carried out by fluorescence imaging. The data were processed with Graphpad Prism software version 4.03. |
| Affinity data for this assay | |
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