| Assay Method Information | |
| | IMAP High-Throughput Screening |
| Description: | The IMAP Screening Express Kit (Molecular Devices, Sunnyvale, CA) was used for the high-throughput screening experiments. Compounds from the Developmental Therapeutics Program (DTP) Open Repository were solubilized and diluted in DMSO and initially tested at one concentration in the assay. Active compounds were subsequently titrated at 20 2-fold dilutions. Reactions were performed using recombinant Chk2 with the indicated drug concentrations in reaction buffer in 384-well black microplate (Greiner Bio-One, Longwood, FL)] for 60 min at room temperature. IMAP binding reagent was added to each well, plates were incubated for 30 min at room temperature, and fluorescence polarization was measured using a Tecan Ultra plate reader. Each screening plate contained staurosporine as a positive control. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |