Assay Method Information | |
| ChEMBL_1755376 (CHEMBL4190136) |
Description: | Inhibition of C-terminal His-tagged recombinant human CYP17A1delta19H mutant expressed in Escherichia coli DH5alpha assessed as decrease in progesterone hydroxylation preincubated for 3 mins followed by NADPH addition measured after 10 mins in presence of cytochrome p450 reductase by LC-MS/MS method |
Affinity data for this assay | |
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