Assay Method Information | |
| ChEMBL_1853066 (CHEMBL4353690) |
Description: | Inhibition of KDM5B (unknown origin) assessed as decrease in demethylation of substrate using peptide (H3(1-21)K4-Me3-GGKBiotin) as substrate and 2OG as cosubstrate pre-incubated for 15 mins before substrate addition followed by centrifugation for 15 secs and measured after 20 mins by Alphascreen assay |
Affinity data for this assay | |
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