| Assay Method Information | |
| | ChEMBL_2067369 (CHEMBL4722622) |
| Description: | Inhibition of C-terminal His-tagged recombinant human MIF tautomerase activity expressed in Escherichia coli BL21 using 4-hydroxyphenyl pyruvic acid as substrate preincubated for 15 mins followed by substrate addition and measured for 10 mins in presence of 0.5 mM EDTA by UV absorbance based Cheng-Prusoff equation analysis |
| Affinity data for this assay | |
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