| Assay Method Information | |
| | ChEMBL_2459658 |
| Description: | Inhibition of N-terminal 6His-tagged human Sirt4 (25 to 314 residues) expressed in Escherichia coli CodonPlus(DE3) cells using HMG-FdL as substrate at 100 uM preincubated for 20 mins followed by NAM addition and measured after 45 mins in presence of NAD+ by fluorescence based assay relative to control |
| Affinity data for this assay | |
|---|---|
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