Assay Method Information

Assay Name:  COX-2 Activity Assay
Description:  Tested compounds (50 uM), DuP-597 (0.5 uM; positive control), 11f and 11eie(1 nM - 200 uM ) or vehicle were pre-incubated with COX-FIS assay buffer (100 mris-HCl, pH 8), 1 uM of COX-FIS heme hCOX-2 FIS assay agent and 30 uM of ADHP (10-acetyl-3,7-dihydroxyphenoxazine) fluorometric substrate for 15 min at room temperature. The reaction was initiated by the addition of arachidonic acid (AA) or AEA to a final concentration of 10 uM. Fluorescence signals were measured after 2 min of incubtion at 535 and 580 nm.
Affinity data for this assay

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