| Assay Method Information | |
| | Isothermal Titration Calorimetry (ITC) |
| Description: | ITC measurements were carried out at 20 °C with a VP-ITC calorimeter (GE Healthcare, MicroCal, Inc.) consisting of a 1.45-ml reaction cell. STM2200 in 20 mM MES-NaOH, 150 mM NaCl, 0.025% (w/v) β-DDM, pH 5.9 at concentrations ranging between 15 and 30 μM was titrated with ligands under conditions where the sum of the ionic strengths of ligand and NaCl was always 150 mM. After an initial 4-μl injection, all subsequent ligand additions consisted of 5 μl every 240-300 s. Stirring was at 307 rpm. |
| Affinity data for this assay | |
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