Assay Method Information

Assay Name:  Mammalian Two-Hybrid Assay
Description:  We performed a mammalian one-hybrid assay in HeLa cells, where we transfected a construct coding for a Gal4DBD-BagN128 fusion or just Gal4DBD alone togetherwith a Gal4 binding site-luciferase reporter gene and an AR expression vector. The transfected cells were then treated with or without DHT, and the reporter gene activity was measured.
Affinity data for this assay

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