| Assay Method Information | |
| | replicon assay |
| Description: | These include a rapid screening replicon assay using baby hamster kidney (BHK) cells with noninfectious SARS-CoV-2, in which the spike protein in the viral genome is replaced with a nanoluciferase reporter, allowing the ability to multiplex in 96-well plates and assess inhibition of viral replication in 24 h. In parallel, the compound general cytotoxicity in either Vero E6 cells or normal human bronchial epithelial (NHBE) cells can be assessed using 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)8,28 or alamarBlue dyes as readouts. |
| Affinity data for this assay | |
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