| Assay Method Information | |
| | In Vitro Assay |
| Description: | Trypsin: In the assay for measuring the inhibition of human trypsin activity buffer (50 mM Tris, pH 8.2, and 20 mM CaCl2) is used. The IC50 is measured by selecting the appropriate wells of a Greiner 384 microtiter plate. 50 μL the assay buffer containing human trypsin (Sigma; T6424), of which the final concentration is 0.39 BAEE units/ml; 2 μL the assay buffer containing 2% (V/V) DMSO (uninhibited control) or various concentrations of the compounds to be tested diluted in the assay buffer containing 2% (V/V) DMSO; and the assay buffer containing substrate S-2222 (Chromogenix). The assay is performed by pre-incubating the tested compounds with enzyme for 10 minutes. Then the assay is initiated by adding 48 μL substrate to obtain a final volume of 100 μL. |
| Affinity data for this assay | |
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