Assay Method Information | |
| ChEMBL_2056242 (CHEMBL4711243) |
Description: | Inhibition of human CKalpha1 assessed as reduction in 14C incorporation from [methyl-14C]choline to phosphatidylcholine using [methyl-14C]choline as substrate preincubated for 5 mins in presence of ATP and MgCl2 followed by substrate addition and measured after 10 mins by liquid scintillation counting method relative to control |
Affinity data for this assay | |
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