| Assay Method Information | |
| | ChEMBL_2546888 |
| Description: | ATP competitive inhibition of recombinant human N-terminal GST-fused CDK4 (S4 to E303 residues)/cyclin D1 expressed in Sf9 insect cells using C-terminal Rb fragment as substrate measured after 50 mins in presence of varying levels of [33P]gamma-ATP by Michaelis-Menten equation based kinetic analysis |
| Affinity data for this assay | |
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