| Assay Method Information | |
| | SPR Directy Binding Assay (Biacore S51) |
| Description: | A concentration series of each compound was injected at a flow rate of 90 µL/min over three spots at 25°C. The times allowed for compound association time and dissociation within the flow cell time were 2 min and 8 min, respectively. The buffer blank was also injected periodically for double referencing along with buffer samples containing an incremental range of 4-6% DMSO, used for solvent correction. The antibody surface was regenerated between binding cycles with 40-s injection of 10 mM glycine-HCl, pH 2.2. The protein was injected at 10 µL/min for 3 min across spot 2 only at the beginning of each cycle. |
| Affinity data for this assay | |
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