| Assay Method Information | |
| | [3H]Mesulergine Binding Assay |
| Description: | Frozen membranes from a stable CHO-K1 cell line expressing the human recombinant 5-HT2C receptor (PerkinElmer) were used. For the binding assay, [3H]mesulergine (1 nM; Amersham Biosciences, Buckinghamshire, UK), receptor membrane, and test compounds were added into 50 mM Tris-HCl (pH 7.4) buffer containing 0.1% ascorbic acid and 4 mM CaCl2. Nonspecific binding was determined using 100 μM mianserin. The incubations were performed for 60 min at 27°C, and these were terminated by rapid filtration through a Filtermat A glass fiber filter presoaked in 0.5% PEI. |
| Affinity data for this assay | |
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