| Assay Method Information | |
| | MMP-3 Activity Assay |
| Description: | The activity of recombinant human MMP-3 (catalytic domain, Calbiochem) was detected with the substrate Mca-Arg-Pro-Lys-Pro-Val-Glu-Nval-Trp-Arg-Lys(Dnp)-NH2 (substrate II, R&D Systems Europe Ltd) (excitation wavelength = 320 nm, emission wavelength = 405 nm). These assays were performed at 37°C in 100 μl assay buffer. The reaction was started by adding 10 μl MMP-3 stock solution (1 ng/μl) to a 100 μl reaction system with a final substrate concentration of 10 μM, after which the rate of substrate hydrolysis was monitored. |
| Affinity data for this assay | |
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