| Assay Method Information | |
| | Radioligand Binding Assay, 5HT2B |
| Description: | Materials and Methods:Receptor Source: Human recombinant 5-HT2B expressed CHO-K1 cellsRadioligand: 1.20 nM [3H] Lysergic acid diethylamide (LSD)Control Compound: KetanserinIncubation Conditions: The reactions were carried out in 50 mM TRIS-HCl (pH 7.6) at room temperature for 90 minutes. The reaction was terminated by rapid vacuum filtration onto glass fiber filters. Radioactivity trapped onto the filters was determined and compared to control values in order to ascertain any interactions of test compounds with the serotonin-5HT2B binding site. |
| Affinity data for this assay | |
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