| Assay Method Information | |
| | TACE Activity Assay |
| Description: | TACE was purchased as an active form enzyme (R&D Systems). Hydrolysis of the TACE-substrate Mca-Pro-Leu-Ala-Gln-Ala-Val-Dpa-Arg-Ser-Ser-Ser-Arg-NH (substrateIII, R&D Systems Europe Ltd) was monitored by measuring the increase in fluorescence (excitation wavelength = 320 nm, emission wavelength = 405 nm). TACEassays were performed at 37°C in 100 μl assay buffer. The reaction was started by adding 4 μl TACE stock solution (1 ng/μl) to a 100 μl reaction system with a final substrate concentration of 10 μM, after which the rate of substrate hydrolysis was monitored. |
| Affinity data for this assay | |
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