| Assay Method Information | |
| | ChEMBL_1656121 (CHEMBL4005591) |
| Description: | Inhibition of recombinant human N-terminal GST-tagged SIRT3 (101 to 399 residues) expressed in Escherichia coli using p53 derived (379 to 382 residues) fluorogenic peptide substrate RHKK(Ac)-AMC) preincubated for 10 mins followed by substrate addition in presence of NAD+ by fluorescence assay |
| Affinity data for this assay | |
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