Assay Method Information | |
| ChEMBL_1656119 (CHEMBL4005589) |
Description: | Inhibition of recombinant human N-terminal His-tagged SIRT1 (1 to 747 end residues) expressed in Escherichia coli using p53 derived (379 to 382 residues) fluorogenic peptide substrate RHKK(Ac)-AMC preincubated for 10 mins followed by substrate addition in presence of NAD+ by fluorescence assay |
Affinity data for this assay | |
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