Assay Method Information

Assay Name:  ChEMBL_1656119 (CHEMBL4005589)
Description:  Inhibition of recombinant human N-terminal His-tagged SIRT1 (1 to 747 end residues) expressed in Escherichia coli using p53 derived (379 to 382 residues) fluorogenic peptide substrate RHKK(Ac)-AMC preincubated for 10 mins followed by substrate addition in presence of NAD+ by fluorescence assay
Affinity data for this assay
 

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