| Assay Method Information | |
| | ChEMBL_2478564 |
| Description: | Inhibition of demethylation of 6xHis-tagged human FTO (32 to 505 residues) extracted from Escherichia coli BL21(DE3) preincubated with compound and 5'-TAGACATTGCCATTCTCGATAGG(6mA)TCCGGTCAAACCTAGACGAATTCCA-3' for 2 hrs followed by addition of FAM-labeled (5'-TGGAATTCGTCTAGGTTTGACCGGATCCTATCGAGAATGGCAATGTCTA-3') by electrophoretic analysis |
| Affinity data for this assay | |
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