Affinity DataKd: 100nMAssay Description:Inhibition of human carbonic anhydrase 4 at pH7 and 37 degC by fluorescent thermal shift assayMore data for this Ligand-Target Pair
Affinity DataKd: 100nMAssay Description:Binding affinity to recombinant human N-terminal His6-tagged carbonic anhydrase 4 expressed in Escherichia coli BL21 (DE3) in presence of ANS by fluo...More data for this Ligand-Target Pair
Affinity DataKd: 14nMAssay Description:Binding affinity to recombinant human N-terminal His6-tagged carbonic anhydrase 4 expressed in Escherichia coli BL21 (DE3) assessed as intrinsic diss...More data for this Ligand-Target Pair
Affinity DataKd: 12nMAssay Description:Binding affinity to recombinant human carbonic anhydrase 4 expressed in Escherichia coli expression system assessed as kinetic dissociation constant ...More data for this Ligand-Target Pair
Affinity DataKd: 87nMAssay Description:Binding affinity to recombinant human carbonic anhydrase 4 expressed in Escherichia coli expression system assessed as observed dissociation constant...More data for this Ligand-Target Pair
Affinity DataIC50: 17.5nMAssay Description:The assay measured the rate of CO2 hydration by determining the addition rate of a NaOH solution using a Radiometer VIT 90 titration system. The enzy...More data for this Ligand-Target Pair
TargetCarbonic anhydrase 1/12/13/14/2/3/4/5A, mitochondrial/5B, mitochondrial/6/7/9(Human)
Istituto Chimico Internazionale Dr. G. Rende
Curated by ChEMBL
Istituto Chimico Internazionale Dr. G. Rende
Curated by ChEMBL
Affinity DataIC50: 440nMAssay Description:Inhibition of Carbonic anhydrase enzyme in rabbitsMore data for this Ligand-Target Pair
TargetCarbonic anhydrase 1/12/13/14/2/3/4/5A, mitochondrial/5B, mitochondrial/6/7/9(Human)
Istituto Chimico Internazionale Dr. G. Rende
Curated by ChEMBL
Istituto Chimico Internazionale Dr. G. Rende
Curated by ChEMBL
Affinity DataKi: 0.800nMAssay Description:Compound was evaluated for the inhibition of Carbonic anhydrase (Non competitive)More data for this Ligand-Target Pair
Affinity DataKi: 8.60nMAssay Description:Inhibition of human CAH4 expressed in Escherichia coli BL21 (DE3) by stopped flow CO2 hydration assayMore data for this Ligand-Target Pair
Affinity DataKi: 11nMAssay Description:Inhibition of human secreted carbonic anhydrase 4 measured by CO2 hydration reaction assayMore data for this Ligand-Target Pair
Affinity DataKi: 25nMAssay Description:Inhibition of recombinant human carbonic anhydrase 4 incubated for 15 mins prior to testing by phenol red based stopped-flow CO2 hydration assayMore data for this Ligand-Target Pair
Affinity DataKi: 70nMAssay Description:Inhibition of recombinant human membrane-associated carbonic anhydrase 4 assessed as inhibition of CO2 hydration preincubated for 15 mins prior to te...More data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:An Applied Photophysics stopped-flow instrument has been used for assaying the CA-catalyzed CO2 hydration activity. Phenol red has been used as indic...More data for this Ligand-Target Pair
Affinity DataKi: 74nM ΔG°: -40.7kJ/molepH: 7.4 T: 2°CAssay Description:An Applied Photophysics stopped-flow instrument has been used for assaying the CA-catalyzed CO2 hydration activity. Phenol red has been used as indic...More data for this Ligand-Target Pair
Affinity DataKi: 74nM ΔG°: -40.4kJ/molepH: 7.5 T: 2°CAssay Description:An Applied Photophysics stopped-flow instrument has been used for assaying the CA-catalyzed CO2 hydration activity. Phenol red has been used as indic...More data for this Ligand-Target Pair
Affinity DataKi: 74nM ΔG°: -37.8kJ/molepH: 7.5 T: 2°CAssay Description:An Applied Photophysics stopped-flow instrument has been used for assaying the CA catalysed CO2 hydration activity. Phenol red (at a concentration of...More data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of human recombinant carbonic anhydrase 4 pre-incubated for 15 mins by stopped-flow CO2 hydration assayMore data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of carbonic anhydrase-4 (unknown origin)More data for this Ligand-Target Pair
Affinity DataKi: 74nM ΔG°: -40.7kJ/moleT: 2°CAssay Description:Inhibition of human recombinant carbonic anhydrase 4 preincubated for 15 mins at room temperature/6 hrs at 4 deg C by stopped-flow CO2 hydration assa...More data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of human recombinant CA4 expressed in Escherichia coli preincubated for 15 mins by stopped-flow CO2 hydration assayMore data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of human recombinant carbonic anhydrase 4 preincubated for 15 mins prior to testing by stopped-flow CO2 hydration assayMore data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of human recombinant carbonic anhydrase 4 expressed in Escherichia coli BL21 (DE3) by stopped-flow CO2 hydration assayMore data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of human carbonic anhydrase 4 by stopped flow CO2 hydration assayMore data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of human carbonic anhydrase 4 by stopped flow CO2 hydration methodMore data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of human carbonic anhydrase 4 after 15 mins by Lineweaver-Burk plotMore data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of human CA4 by CO2 hydration assayMore data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of human recombinant carbonic anhydrase 4 preincubated for 15 mins by CO2 hydration methodMore data for this Ligand-Target Pair
Affinity DataKi: 74nMpH: 7.5Assay Description:Inhibition of human carbonic anhydrase 4 preincubated for 15 mins at pH 7.5 by stopped flow CO2 hydration methodMore data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibitory activity against human carbonic anhydrase IV (hCAIV)More data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of human recombinant CA4 after 15 mins by CO2 hydration stopped flow assayMore data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of human carbonic anhydrase 4More data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of human recombinant CA4 by stopped flow CO2 hydrase assayMore data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of human recombinant full length CA4 by stopped flow CO2 hydration assayMore data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of human CA4 by stopped-flow CO2 hydration assayMore data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of human full length carbonic anhydrase 4 by stopped flow CO2 hydrase assayMore data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of truncated human cloned membrane-associated carbonic anhydrase 4 preincubated for 15 mins by stopped-flow CO2 hydration assayMore data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of human recombinant carbonic anhydrase 4 expressed in Escherichia coli assessed as reduction in CO2 hydration preincubated for 15 mins pr...More data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of human recombinant carbonic anhydrase 4 after 15 mins by stopped-flow CO2 hydration assayMore data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of full length human carbonic anhydrase 4 preincubated for 15 mins by CO2 hydration stopped-flow assayMore data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of recombinant human carbonic anhydrase 4 assessed as reduction in CO2 hydration preincubated for 15 mins by stopped flow assayMore data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of human recombinant carbonic anhydrase-4 using CO2 as substrate preincubated for 10 mins by stopped-flow CO2 hydrase assayMore data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of recombinant human carbonic anhydrase-4 assessed as reduction in enzyme-catalyzed hydration activity using CO2 as substrate preincubated...More data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of recombinant human CA4 preincubated for 15 mins by stopped-flow CO2 hydration assayMore data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of recombinant human carbonic anhydrase 4 incubated for 10 mins prior to testing by stopped flow CO2 hydrase assayMore data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of human recombinant carbonic anhydrase 4 incubated for 15 mins prior to testing by stopped-flow CO2 hydration assayMore data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of recombinant human membrane bound carbonic anhydrase 4 pretreated for 15 mins prior to testing measured for 10 to 100 secs by phenol red...More data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of human CA4 by stopped-flow CO2 hydrase assayMore data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of human carbonic anhydrase 4 incubated for 15 mins by stopped flow CO2 hydrase assayMore data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Inhibition of human membrane-anchored carbonic anhydrase 4 assessed as inhibition of CO2 hydration preincubated for 15 mins prior to testing by pheno...More data for this Ligand-Target Pair
Affinity DataKi: 74nMAssay Description:Binding affinity to human carbonic anhydrase 4 by stopped flow CO2 hydration assayMore data for this Ligand-Target Pair

3D Structure (crystal)