Affinity DataEC50: 3.75E+4nMAssay Description:Inhibition of USP7 in human SJSA cells assessed as increase in ubiquitinated MDM2 level after 16 hrs in presence of MG132 by MSD assayMore data for this Ligand-Target Pair
Affinity DataEC50: 3.65E+4nMAssay Description:Inhibition of USP7 in human SJSA cells assessed as increase in ubiquitinated MDM2 level after 16 hrs in presence of MG132 by MSD assayMore data for this Ligand-Target Pair
Affinity DataEC50: 2.58E+3nMAssay Description:Inhibition of USP7 in human SJSA cells assessed as increase in ubiquitinated MDM2 level after 16 hrs in presence of MG132 by MSD assayMore data for this Ligand-Target Pair
Affinity DataEC50: >5.00E+4nMAssay Description:Inhibition of USP7 in human SJSA cells assessed as increase in ubiquitinated MDM2 level after 16 hrs in presence of MG132 by MSD assayMore data for this Ligand-Target Pair
Affinity DataKd: 1.20E+4nMAssay Description:Binding affinity to human His-tagged and [15N-13C-2H], delta1[13CH3]-Ile, [13CH3]-Leu/Val and [13CH3]-Met-labeled USP7-catalytic domain (208 to 554 r...More data for this Ligand-Target Pair
Affinity DataEC50: 300nMAssay Description:Inhibition of USP7 in human SJSA cells assessed as increase in ubiquitinated MDM2 level after 16 hrs in presence of MG132 by MSD assayMore data for this Ligand-Target Pair
Affinity DataKd: 1.20E+4nMAssay Description:Binding affinity to human His-tagged and [15N-13C-2H], delta1[13CH3]-Ile, [13CH3]-Leu/Val and [13CH3]-Met-labeled USP7-catalytic domain (208 to 554 r...More data for this Ligand-Target Pair
Affinity DataKd: 4.71E+5nMAssay Description:Binding affinity to recombinant human N-terminal His6-tagged USP7 catalytic domain (213 to 548 residues) expressed in baculovirus infected Sf21 insec...More data for this Ligand-Target Pair
Affinity DataEC50: 320nMAssay Description:Inhibition of USP7 in human HCT116 cells using ubiquitin-propargylamine probe as substrate incubated for 2 hrs followed by substrate addition measure...More data for this Ligand-Target Pair
3D Structure (crystal)
Affinity DataEC50: 250nMAssay Description:Inhibition of USP7 in human RKO cells transfected with p53 luciferase reporter vector assessed as increase in p53-dependent luciferase activity measu...More data for this Ligand-Target Pair
Affinity DataEC50: 33nMAssay Description:Inhibition of USP7 in human RKO cells transfected with p53 luciferase reporter vector assessed as increase in p53-dependent luciferase activity measu...More data for this Ligand-Target Pair
Affinity DataEC50: 130nMAssay Description:Inhibition of USP7 in human RKO cells transfected with p53 luciferase reporter vector assessed as increase in p53-dependent luciferase activity measu...More data for this Ligand-Target Pair
Affinity DataEC50: 560nMAssay Description:Inhibition of USP7 in human RKO cells transfected with p53 luciferase reporter vector assessed as increase in p53-dependent luciferase activity measu...More data for this Ligand-Target Pair
Affinity DataEC50: 180nMAssay Description:Inhibition of USP7 in human RKO cells transfected with p53 luciferase reporter vector assessed as increase in p53-dependent luciferase activity measu...More data for this Ligand-Target Pair
Affinity DataEC50: 400nMAssay Description:Inhibition of USP7 in human RKO cells transfected with p53 luciferase reporter vector assessed as increase in p53-dependent luciferase activity measu...More data for this Ligand-Target Pair
Affinity DataEC50: 320nMAssay Description:Inhibition of USP7 in human RKO cells transfected with p53 luciferase reporter vector assessed as increase in p53-dependent luciferase activity measu...More data for this Ligand-Target Pair
Affinity DataEC50: 150nMAssay Description:Inhibition of USP7 in human RKO cells transfected with p53 luciferase reporter vector assessed as increase in p53-dependent luciferase activity measu...More data for this Ligand-Target Pair
Affinity DataEC50: 150nMAssay Description:Inhibition of USP7 in human RKO cells transfected with p53 luciferase reporter vector assessed as increase in p53-dependent luciferase activity measu...More data for this Ligand-Target Pair
Affinity DataEC50: 170nMAssay Description:Inhibition of USP7 in human RKO cells transfected with p53 luciferase reporter vector assessed as increase in p53-dependent luciferase activity measu...More data for this Ligand-Target Pair
Affinity DataEC50: 65nMAssay Description:Inhibition of USP7 in human RKO cells transfected with p53 luciferase reporter vector assessed as increase in p53-dependent luciferase activity measu...More data for this Ligand-Target Pair
Affinity DataEC50: 42nMAssay Description:Inhibition of USP7 in human RKO cells transfected with p53 luciferase reporter vector assessed as increase in p53-dependent luciferase activity measu...More data for this Ligand-Target Pair
Affinity DataEC50: 1.20E+3nMAssay Description:Inhibition of USP7 in human RKO cells transfected with p53 luciferase reporter vector assessed as increase in p53-dependent luciferase activity measu...More data for this Ligand-Target Pair
Affinity DataEC50: 280nMAssay Description:Inhibition of USP7 in human RKO cells transfected with p53 luciferase reporter vector assessed as increase in p53-dependent luciferase activity measu...More data for this Ligand-Target Pair
Affinity DataEC50: 60nMAssay Description:Inhibition of USP7 in human RKO cells transfected with p53 luciferase reporter vector assessed as increase in p53-dependent luciferase activity measu...More data for this Ligand-Target Pair
Affinity DataEC50: 35nMAssay Description:Inhibition of USP7 in human RKO cells transfected with p53 luciferase reporter vector assessed as increase in p53-dependent luciferase activity measu...More data for this Ligand-Target Pair
Affinity DataEC50: 210nMAssay Description:Inhibition of USP7 in human RKO cells transfected with p53 luciferase reporter vector assessed as increase in p53-dependent luciferase activity measu...More data for this Ligand-Target Pair
Affinity DataEC50: 60nMAssay Description:Inhibition of USP7 in human RKO cells transfected with p53 luciferase reporter vector assessed as increase in p53-dependent luciferase activity measu...More data for this Ligand-Target Pair
Affinity DataEC50: 25nMAssay Description:Inhibition of USP7 in human RKO cells transfected with p53 luciferase reporter vector assessed as increase in p53-dependent luciferase activity measu...More data for this Ligand-Target Pair
Affinity DataKd: 78nMAssay Description:Binding affinity to USP7 (208 to 560 residues) (unknown origin) assessed as dissociation constant by biolayer interferometryMore data for this Ligand-Target Pair
Affinity DataKd: 42nMAssay Description:Binding affinity to USP7 (208 to 560 residues) (unknown origin) assessed as dissociation constant by biolayer interferometryMore data for this Ligand-Target Pair
Affinity DataKd: 6.80E+5nMAssay Description:Allosteric binding affinity to USP7 catalytic domain (unknown origin) at Met328, Trp285, Gly284, Gln405 and Gly313 residues by NMR-15N-1H TROSY spect...More data for this Ligand-Target Pair
Affinity DataKd: 5.80E+5nMAssay Description:Allosteric binding affinity to USP7 catalytic domain (unknown origin) at Met328 residues by NMR-15N-1H TROSY spectral titration analysisMore data for this Ligand-Target Pair
Affinity DataKd: 5.40E+5nMAssay Description:Binding affinity to USP7 (unknown origin) by T2 relaxation experiment based analysisMore data for this Ligand-Target Pair
Affinity DataKd: 7.70E+4nMAssay Description:Binding affinity to recombinant USP7 (208 to 560 residues) (unknown origin) assessed as dissociation constant by biolayer interferometryMore data for this Ligand-Target Pair
Affinity DataKd: 128nMAssay Description:Binding affinity to human USP7 (208 to 560 residues) by SPR methodMore data for this Ligand-Target Pair
Affinity DataKd: 86nMAssay Description:Binding affinity to human USP7 (208 to 560 residues) by SPR methodMore data for this Ligand-Target Pair
Affinity DataKd: 92nMAssay Description:Binding affinity to human USP7 (208 to 560 residues) by SPR methodMore data for this Ligand-Target Pair
Affinity DataKd: 120nMAssay Description:Binding affinity to human USP7 (208 to 560 residues) by SPR methodMore data for this Ligand-Target Pair
Affinity DataKd: 675nMAssay Description:Binding affinity to human USP7 (208 to 560 residues) by SPR methodMore data for this Ligand-Target Pair
Affinity DataKd: 541nMAssay Description:Binding affinity to human USP7 (208 to 560 residues) by SPR methodMore data for this Ligand-Target Pair
Affinity DataKd: 148nMAssay Description:Binding affinity to human USP7 (208 to 560 residues) by SPR methodMore data for this Ligand-Target Pair
Affinity DataKd: 53nMAssay Description:Binding affinity to human USP7 (208 to 560 residues) by SPR methodMore data for this Ligand-Target Pair
Affinity DataEC50: 6.90E+3nMAssay Description:Inhibition of USP7 (unknown origin)More data for this Ligand-Target Pair
Affinity DataKd: 4.71E+5nMAssay Description:Inhibition of human USP7 using Ub-Rho110 as substrate assessed as dissociation constant by fluorescence based SPR analysisMore data for this Ligand-Target Pair
Affinity DataKd: 4.71E+5nMAssay Description:Inhibition of human USP7 using Ub-Rho110 as substrate assessed as dissociation constant by fluorescence based SPR analysisMore data for this Ligand-Target Pair
Affinity DataKd: 7.80E+3nMAssay Description:Inhibition of human his tagged USP7 (208 to 560 residues) expressed in insect sf9 cells using monoubiquitinated ubiquitin-rhodamine as substrate asse...More data for this Ligand-Target Pair
Affinity DataEC50: 220nMAssay Description:Inhibition of human USP7 using Ub-EKL as substrate by SDS-PAGE assayMore data for this Ligand-Target Pair
Affinity DataEC50: 640nMAssay Description:Inhibition of human USP7 using Ub-EKL as substrate by SDS-PAGE assayMore data for this Ligand-Target Pair
Affinity DataEC50: 790nMAssay Description:Inhibition of human USP7 using Ub-EKL as substrate by SDS-PAGE assayMore data for this Ligand-Target Pair
Affinity DataEC50: 2.00E+3nMAssay Description:Inhibition of human USP7 using Ub-EKL as substrate by SDS-PAGE assayMore data for this Ligand-Target Pair
