BDBM50396483 PLX-4032::RG 7204::Ro 5185426::US10570155, Vemurafenib::US11332479, Compound Vemurafenib::US11492357, Control::US9388165, Reference, Vemurafenib (PLX-4032)::USRE47451, PLX-4032, Roche::VEMURAFENIB
SMILES CCCS(=O)(=O)Nc1ccc(F)c(C(=O)c2c[nH]c3ncc(cc23)-c2ccc(Cl)cc2)c1F
InChI Key InChIKey=GPXBXXGIAQBQNI-UHFFFAOYSA-N
Activity Spreadsheet -- Enzyme Inhibition Constant Data from BindingDB
Found 108 hits for monomerid = 50396483
Affinity DataIC50: 2.10nMAssay Description:Inhibition of ARAF (unknown origin) using human His6-tagged MEK1 K97R mutant as substrate pretreated for 20 mins followed by [33P]-ATP addition measu...More data for this Ligand-Target Pair
Affinity DataIC50: 2.10nMAssay Description:Inhibition of ARaf (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 4nMAssay Description:Inhibition of B-Raf V600E mutant (unknown origin) assessed as MEK1 phosphorylation using MEK1-Avitag as substrate after 1 hr by HTRF assayMore data for this Ligand-Target Pair
TargetMitogen-activated protein kinase kinase kinase 20(Human)
Johann Wolfgang Goethe University
Curated by ChEMBL
Johann Wolfgang Goethe University
Curated by ChEMBL
Affinity DataIC50: 4nMAssay Description:Inhibition of full-length human ZAK using MBP as substrate in presence off ATP by competitive binding assayMore data for this Ligand-Target Pair
Affinity DataIC50: 6.10nMAssay Description:Inhibition of recombinant BRAF V600E mutant (unknown origin) assessed as ADP formation measured for 5 hrs by pyruvate kinase/lactate dehydrogenase co...More data for this Ligand-Target Pair
Affinity DataIC50: 6.90nMAssay Description:Inhibition of BRAF (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 6.90nMAssay Description:Inhibition of wild-type BRAF (unknown origin) using human His6-tagged MEK1 K97R mutant as substrate pretreated for 20 mins followed by [33P]-ATP addi...More data for this Ligand-Target Pair
TargetDual specificity mitogen-activated protein kinase kinase 4(Mouse)
Eberhard Karls Universit£T T£Bingen
Curated by ChEMBL
Eberhard Karls Universit£T T£Bingen
Curated by ChEMBL
Affinity DataKd: 8.20nMAssay Description:Binding affinity to GST tagged mouse MKK4 expressed in Escherichia coli expression system measured by Kinomescan assayMore data for this Ligand-Target Pair
Affinity DataIC50: 9.60nMAssay Description:Inhibition of human recombinant BRAF V600E mutant using MEK1 as substrate measured after 1 hr by Western blot analysisMore data for this Ligand-Target Pair
Affinity DataKd: 10nMAssay Description:Binding affinity to BRAF V600E mutant in human WM278 cells by KINOMEscann Kinase binding assayMore data for this Ligand-Target Pair
Affinity DataIC50: 13nMAssay Description:Inhibition of B-Raf V600E mutant (448 to 723 residues) (unknown origin) expressed in Escherichia coli by AlphaScreen assayMore data for this Ligand-Target Pair
Affinity DataIC50: 13nMAssay Description:Inhibition of wild type N-terminal 6His-tagged BRAF V600E mutant (unknown origin) (D448 to K723 residues) expressed in baculovirus expression system ...More data for this Ligand-Target Pair
Affinity DataIC50: 17nMAssay Description:Inhibition of B-Raf V600E mutant in human A375 cells assessed as ERK phosphorylation preincubated for 1 hr by Western blot methodMore data for this Ligand-Target Pair
TargetActivated CDC42 kinase 1(Human)
National Clinical Research Center For Geriatrics
Curated by ChEMBL
National Clinical Research Center For Geriatrics
Curated by ChEMBL
Affinity DataIC50: 19nMAssay Description:Inhibition of ACK1 (unknown origin)More data for this Ligand-Target Pair
TargetActivated CDC42 kinase 1(Human)
National Clinical Research Center For Geriatrics
Curated by ChEMBL
National Clinical Research Center For Geriatrics
Curated by ChEMBL
Affinity DataIC50: 19nMAssay Description:Inhibition of ACK1 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 20nMAssay Description:Inhibition of wild type B-Raf (unknown origin) assessed as MEK1 phosphorylation using MEK1-Avitag as substrate after 1 hr by HTRF assayMore data for this Ligand-Target Pair
Affinity DataIC50: 21nMAssay Description:Inhibition of recombinant His-tagged BRAF V600E mutant (unknown origin) (444-723 residues) expressed in Escherichia coli BL21(DE3)-RIL using MEKK97R ...More data for this Ligand-Target Pair
Affinity DataIC50: 21nMAssay Description:Inhibition of wild type BRAF (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 21nMAssay Description:Inhibition of BRAF V600E mutant (unknown origin) after 20 mins by immunoblotting assayMore data for this Ligand-Target Pair
Affinity DataIC50: 23nMAssay Description:Inhibition of human GST-tagged full length B-Raf V600E mutant (unknown origin) using His6-tagged full-length human MEK1 (K97R) substrate and [33P]-ga...More data for this Ligand-Target Pair
TargetMitogen-activated protein kinase kinase kinase 20(Human)
Johann Wolfgang Goethe University
Curated by ChEMBL
Johann Wolfgang Goethe University
Curated by ChEMBL
Affinity DataIC50: 23nMAssay Description:Inhibition of human ZAK (5 to 309 residues) expressed in baculovirus infected Sf9 insect cells using ZAKtide as substrate after 1 hr by mass spectrom...More data for this Ligand-Target Pair
Affinity DataIC50: 30nMAssay Description:Inhibition of mouse full length GST-tagged BRAF V600E mutant using recombinant human full length N-terminal His-tagged MEK1 as substrate preincubated...More data for this Ligand-Target Pair
Affinity DataIC50: 31nMAssay Description:Inhibition of full length human B-Raf V600E mutant expressed in baculovirus infected insect cells assessed as [gamma-33P]incorporation into MEK after...More data for this Ligand-Target Pair
Affinity DataIC50: 31nMAssay Description:Inhibition of recombinant human GST-tagged BRAF V600E mutant expressed in baculovirus expression systemMore data for this Ligand-Target Pair
Affinity DataIC50: 31nMAssay Description:Inhibition of N-terminal his-tagged BRAF V600E mutant (448 to 723 residues) (unknown origin) expressed in Escherichia coli BL21(DE3) cells assessed a...More data for this Ligand-Target Pair
Affinity DataIC50: 31nMAssay Description:Inhibition of BRAF V600E mutant (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 31nMAssay Description:Inhibition of human BRAF V600E mutant using MEK1 as substrate in presence of ATPMore data for this Ligand-Target Pair
TargetMitogen-activated protein kinase kinase kinase 20(Human)
Johann Wolfgang Goethe University
Curated by ChEMBL
Johann Wolfgang Goethe University
Curated by ChEMBL
Affinity DataIC50: 31nMAssay Description:Inhibition of N-terminal GST-tagged recombinant human full-length ZAK expressed in baculovirus infected Sf9 insect cells using MBP as substrate after...More data for this Ligand-Target Pair
Affinity DataIC50: 31nMAssay Description:Inhibition of B-Raf (unknown origin)More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf [V600E](Human)
Korea Institute of Science and Technology
US Patent
Korea Institute of Science and Technology
US Patent
Affinity DataIC50: 31nMAssay Description:Reaction biology kinase hotspot service (http://www.reactionbiology.com) was used to measure IC50. In a final reaction volume of 25 μL, kinase (...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf [V600E](Human)
Korea Institute of Science and Technology
US Patent
Korea Institute of Science and Technology
US Patent
Affinity DataIC50: 31nMAssay Description:Reaction biology kinase hotspot service (http://www.reactionbiology.com) was used to measure IC50. In a final reaction volume of 25 μL, kinase (...More data for this Ligand-Target Pair
Affinity DataIC50: 31nMAssay Description:Inhibition of BRAF (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 31nMAssay Description:Inhibition of N-terminal His-tagged BRAF V600E mutant (unknown origin) expressed in baculovirus infected insect cells co-expressing CDC37 using bioti...More data for this Ligand-Target Pair
Affinity DataIC50: 32nMAssay Description:Inhibition of BRAF V600E mutant in human A375 cells assessed as reduction in ERK phosphorylation by AlphaScreen assayMore data for this Ligand-Target Pair
Affinity DataIC50: 32nMAssay Description:Inhibition of human GST-tagged BRAF V600E mutant (416 to 766 residues) using human full length 6His-tagged MEK1 (K97R) as substrate preincubated for ...More data for this Ligand-Target Pair
Affinity DataIC50: 33nMAssay Description:Inhibition of human recombinant B-Raf V600E mutant by FRET-based Z'-lyte assayMore data for this Ligand-Target Pair
Affinity DataIC50: 33nMAssay Description:Inhibition of B-raf V600E mutant in human A375 cells assessed as reduction in ERK1/2 phosphorylation incubated for 90 mins by Western blotting methodMore data for this Ligand-Target Pair
Affinity DataIC50: 34nMAssay Description:Inhibition of wild type BRAF (unknown origin) assessed as ADP formation measured for 5 hrs by pyruvate kinase/lactate dehydrogenase coupled assay in ...More data for this Ligand-Target Pair
Affinity DataIC50: 48nMAssay Description:Inhibition of C-Raf in presence of 100 uM ATPMore data for this Ligand-Target Pair
TargetEpidermal growth factor receptor [696-1022,L858R](Human)
Korea Institute of Science and Technology
US Patent
Korea Institute of Science and Technology
US Patent
Affinity DataIC50: 48nMAssay Description:Reaction biology kinase hotspot service (http://www.reactionbiology.com) was used to measure IC50. In a final reaction volume of 25 μL, kinase (...More data for this Ligand-Target Pair
Affinity DataIC50: 48nMAssay Description:Reaction biology kinase hotspot service (http://www.reactionbiology.com) was used to measure IC50. In a final reaction volume of 25 μL, kinase (...More data for this Ligand-Target Pair
Affinity DataKd: 51nMAssay Description:Binding affinity to DNA-tagged BRAF (unknown origin) by KINOMEscan assayMore data for this Ligand-Target Pair
Affinity DataKd: 58nMAssay Description:Binding affinity to DNA-tagged BRAF (unknown origin) V600E mutant by KINOMEscan assayMore data for this Ligand-Target Pair
Affinity DataIC50: 59nMAssay Description:Inhibition of recombinant human N-terminal GST-tagged B-Raf V600E mutant expressed in baculovirus expression system using Z'-LYTE Ser/Thr3 peptide as...More data for this Ligand-Target Pair
Affinity DataIC50: 61nMAssay Description:Inhibition of B-Raf V600E mutant-mediated Erk phosphorylation in human MALME-3M cells after 1 hr by fluorescence analysisMore data for this Ligand-Target Pair
Affinity DataIC50: 62nMAssay Description:Inhibition of B-RAF V600E mutant (unknown origin) expressed in Escherichia coli BL21 (DE3)More data for this Ligand-Target Pair
