Query String: Hemin
BDBM512906 Hemin
Hemin BDBM231681
- ChEBML_1716251 Inhibition of ovine COX1 catalytic activity using arachidonic acid as substrate preincubated for 5 mins followed by substrate and hemin addition measured after 2 mins by colorimetric method
- ChEBML_1716252 Inhibition of recombinant ovine COX2 catalytic activity using arachidonic acid as substrate preincubated for 5 mins followed by substrate and hemin addition measured after 2 mins by colorimetric method
- ChEBML_1733252 Inhibition of HO-1 in Sprague-Dawley albino rat spleen microsomes assessed as reduction in bilirubin formation using hemin as substrate after 60 mins in presence of NADPH by biliverdin reductase enzyme coupled spectrophotometric assay
- ChEBML_1733253 Inhibition of HO-2 in Sprague-Dawley albino rat brain microsomes assessed as reduction in bilirubin formation using hemin as substrate after 60 mins in presence of NADPH by biliverdin reductase enzyme coupled spectrophotometric assay
- ChEMBL_1733252 (CHEMBL4148788) Inhibition of HO-1 in Sprague-Dawley albino rat spleen microsomes assessed as reduction in bilirubin formation using hemin as substrate after 60 mins in presence of NADPH by biliverdin reductase enzyme coupled spectrophotometric assay
- Heme Binding Assay Heme binding was tracked by difference spectroscopy in the Soret region of the UV−visible spectrum. Successive aliquots of 0.5 mM hemin in 0.1 M NaOH were added to both the sample cuvette, which contained 10 μM apo-HutZ, and the referencecuvette. Spectra were recorded 3 min after the addition of each heme aliquot.
- Cox-1 Assay The effect of the compounds on COX-1 activity was determined by a continuous colorimetric assay that monitored the oxidation of N, N, N, N-tetramethyl-1,4-phenylenediamine (TMPD) when coupled to the COX catalyzed formation of PGH2 from PGG2 using arachidonic acid as substrate. In brief, 200 μL of reaction solution was composed of 100 mM Tris-HCl (pH 8.0), 2 μM Hemin (Sigma), 5% DMSO, a serial dilution of compounds, COX-1 enzyme (175 nM), 80 μM TMPD (Sigma), and 20 μM arachidonic acid (Sigma). Reagents were mixed and incubated at 25° C. for 5 min followed by adding a mixture of TMPD and arachidonic acid to initiate the reaction. A continuous colorimetric assay to measuring TMPD oxidation at 610 nm was conducted using a Synergy 2 plate reader at 25° C. for 5 min, and IC50 value of each compound was calculated (Adeniji, et al., 2012, J Med Chem 55:2311-2323).