Simple Search Results

ChEMBL_2430724 Inhibition of hypoxia induced VEGFR-2 (unknown origin)
ChEMBL_750098 (CHEMBL1787515) Inhibition of hypoxia-induced HIF1alpha transcriptional activity in HEK293 cells incubated for 16 hrs by hypoxia response element-driven luciferase reporter gene assay
ChEMBL_481746 (CHEMBL1002847) Inhibition of hypoxia-induced HIF1 activation in human U251 cells
ChEMBL_321108 (CHEMBL882874) Effective concentration to inhibit production of hypoxia inducible factor 1-alpha protein
ChEMBL_475987 (CHEMBL931441) Inhibition of hypoxia-induced HIF1 activation in human AGS cells by reporter gene assay
ChEMBL_527926 (CHEMBL975114) Inhibition of hypoxia-induced HIF1 activation in human T47D cells by reporter gene assay
ChEMBL_527928 (CHEMBL975116) Inhibition of hypoxia-induced HIF1 activation in human PC3 cells by reporter gene assay
ChEMBL_767399 (CHEMBL1825511) Inhibition of hypoxia-induced HIF1 activation in human LN229 cells expressing HRE-AP reporter gene preincubated for 1 hr under normoxia condition followed by 24 hrs incubation under hypoxia condition by HRE-luciferase reporter assay
ChEMBL_1294789 (CHEMBL3128876) Inhibition of Stat3 phosphorylation in human OVCAR3 cells by Western blotting analysis in hypoxia condition
ChEMBL_1294791 (CHEMBL3128878) Inhibition of Stat3 phosphorylation in human PANC1 cells by Western blotting analysis in hypoxia condition
ChEMBL_550371 (CHEMBL999925) Inhibition of hypoxia-induced HIF1 activation in human HeLa cells by luciferase reporter gene assay
ChEMBL_1294796 (CHEMBL3128919) Inhibition of HIF-1 alpha in human OVCAR3 cells by Western blotting analysis in hypoxia condition
ChEMBL_1294797 (CHEMBL3128920) Inhibition of HIF-1 alpha in human PANC1 cells by Western blotting analysis in hypoxia condition
ChEMBL_458038 (CHEMBL924307) Inhibition of hypoxia induced HIF1-alpha transcriptional activity in human Hep3B cells by reporter gene assay
ChEMBL_458039 (CHEMBL924308) Inhibition of hypoxia induced HIF1-alpha transcriptional activity in human AGS cells by reporter gene assay
ChEMBL_469602 (CHEMBL933161) Inhibition of hypoxia-induced HIF1 transcriptional activation in human U251-HRE cells by luciferase reporter assay
ChEMBL_478428 (CHEMBL932617) Inhibition of hypoxia-induced HIF1 activation in human T47D cells by cell based reporter gene assay
ChEMBL_1294792 (CHEMBL3128879) Inhibition of Stat3 phosphorylation in human MDA-MB-468 cells by Western blotting analysis in hypoxia condition
ChEMBL_1294793 (CHEMBL3128880) Inhibition of Stat3 phosphorylation in human MDA-MB-231 cells by Western blotting analysis in hypoxia condition
ChEMBL_2281079 Inhibition of hypoxia-induced HIF1alpha/P300 interaction (unknown origin) incubated for 30 mins for fluorescence anisotropy competition assay
ChEMBL_428795 (CHEMBL916228) Inhibition of hypoxia induced HIF1 transcriptional activity in human Hep3B cells by cell-based HRE reporter assay
ChEMBL_458037 (CHEMBL924306) Inhibition of hypoxia induced HIF1-alpha transcriptional activity in human Sk-Hep1 cells by reporter gene assay
ChEMBL_573857 (CHEMBL1056199) Inhibition of hypoxia-induced HIF1 activation in human Hep3B cells by pGL3-HRE-luciferase reporter gene assay
ChEMBL_619005 (CHEMBL1101830) Inhibition of hypoxia-induced HIF1 activation in human T47D cells by pTK-HRE3-luciferase reporter gene assay
ChEMBL_698465 (CHEMBL1647397) Inhibition of hypoxia-induced HIF1 activation in human T47D cells by HRE3-TK-luciferase reporter gene assay
ChEMBL_698468 (CHEMBL1647400) Inhibition of hypoxia-induced HIF1 activation in human PC3 cells by HRE3-TK-luciferase reporter gene assay
ChEMBL_770820 (CHEMBL1837625) Inhibition of hypoxia-induced HIF1alpha activation in human ME180 cells by HRE3-Bla-luciferase reporter gene assay
ChEMBL_881925 (CHEMBL2212528) Inhibition of HIF-1alpha in human HCT116 cells under hypoxia condition by HRE-luciferase reporter gene assay
ChEMBL_881926 (CHEMBL2212529) Inhibition of HIF-1alpha in human LN229 cells under hypoxia condition by HRE-luciferase reporter gene assay
ChEMBL_1294798 (CHEMBL3128921) Inhibition of HIF-1 alpha in human MDA-MB-468 cells by Western blotting analysis in hypoxia condition
ChEMBL_1294799 (CHEMBL3128922) Inhibition of HIF-1 alpha in human MDA-MB-231 cells by Western blotting analysis in hypoxia condition
ChEMBL_452324 (CHEMBL901549) Inhibition of hypoxia-induced HIF1 activation in human T47D cells after 16 hrs by cell based luciferase assay
ChEMBL_550357 (CHEMBL999911) Inhibition of hypoxia-induced HIF1 activation in human T47D cells after 16 hrs by luciferase reporter gene assay
ChEMBL_753564 (CHEMBL1799182) Inhibition of hypoxia-induced HIF1alpha activation in human Hep3B cells after 16 hrs by HRE-luciferase reporter assay
ChEBML_1685544 Inhibition of hypoxia-induced HIF1alpha (unknown origin) transcriptional activity expressed in human HCT116 cells by HRE luciferase reporter gene assay
ChEMBL_610892 (CHEMBL1065047) Inhibition of hypoxia-induced HIF1alpha activation in human HeLa cells after 12 hrs by HRE-luciferase reporter gene assay
ChEMBL_1627457 (CHEMBL3869978) Inhibition of hypoxia-induced HIF1 transcriptional activity in human HeLa cells measured after 12 hrs by luciferase reporter gene assay
ChEMBL_481732 (CHEMBL1002833) Inhibition of hypoxia-induced HIF1 activation in human T47D cells carrying pTK-HER3-luc reporter gene measured by luciferase activity
ChEMBL_526045 (CHEMBL967263) Inhibition of hypoxia-induced HIF1 activation in human AGS cells after 16 hrs by pGL3-HRE-luciferase reporter gene assay
ChEMBL_549581 (CHEMBL1015932) Inhibition of hypoxia-induced HIF1 activation in human T47D cells after 16 hrs by pTK-HRE3-luciferase reporter gene assay
ChEMBL_621709 (CHEMBL1104951) Inhibition of hypoxia-induced HIF1 activation in human PC3 cells after 16 hrs by pTK-HRE3-luciferase reporter gene assay
ChEMBL_621727 (CHEMBL1108459) Inhibition of hypoxia-induced HIF1 activation in human T47D cells after 16 hrs by pTK-HRE3-luciferase reporter gene assay
ChEMBL_621731 (CHEMBL1108463) Inhibition of hypoxia-induced HIF1 activation in human T47D cells expressing pGL3 construct after 16 hrs by reporter gene assay
ChEMBL_776653 (CHEMBL1913750) Inhibition of hypoxia-induced HIF1 activation in human T47D cells after 16 hrs by HRE3-TK-luciferase reporter gene assay
ChEMBL_851302 (CHEMBL2155888) Inhibition of hypoxia-induced HIF1 activation in human T47D cells after 16 hrs by HRE3-TK-luciferase reporter gene assay
ChEMBL_2288784 Inhibition of DHODH in human HeLa cells assessed as reduction in hypoxia-induced VEGF expression incubated for 48 hrs by ELISA assay
ChEMBL_631037 (CHEMBL1113762) Inhibition of hypoxia-induced HIF1 activation in human T47D cells in presence of 1% O2 by HRE3-TK-luc reporter assay
ChEMBL_2375999 Inhibition of HIF-1 alpha transcriptional activity in human U-251 cells incubated for 24 hrs under hypoxia condition by luciferse reporter assay
ChEMBL_439940 (CHEMBL890257) Inhibition of HIF1 activation in human AGS cells assessed as inhibition of hypoxia-induced luciferase expression after 16 hrs by reporter assay
ChEMBL_439941 (CHEMBL890258) Inhibition of HIF1 activation in human Hep3B cells assessed as inhibition of hypoxia-induced luciferase expression after 16 hrs by reporter assay
ChEMBL_735620 (CHEMBL1693322) Inhibition of hypoxia-induced HIF1 activation in human T47D cells expressing pGL3 construct after 16 hrs by cell-based luciferase reporter assay
ChEMBL_938618 (CHEMBL2327238) Inhibition of hypoxia-induced HIF1alpha transcriptional activity in human HeLa cells expressing HRE-Luc after 12 hrs by luciferase reporter gene assay
Evaluation of Inhibitory Activity Against Retinal Neovascularization by Hypoxia The experiment was performed in order to evaluate inhibitory activity against angiogenesis of the compounds.
ChEMBL_573855 (CHEMBL1055397) Inhibition of hypoxia-induced HIF1alpha protein accumulation in human Hep3B cells treated for 30 mins measured after 12 hrs by Western blot analysis
ChEMBL_631036 (CHEMBL1113761) Inhibition of chemical hypoxia-induced HIF1 activation in human T47D cells in presence of 1, 10-phenanthrolin by HRE3-TK-luc reporter assay
ChEMBL_636313 (CHEMBL1169036) Inhibition of hypoxia-induced HIF1alpha protein accumulation in human Hep3B cells treated for 30 mins measured after 12 hrs by Western blot analysis
ChEMBL_1855371 (CHEMBL4356100) Inhibition of HIF1alpha transcriptional activity in hypoxia-induced human HCCLM3 cells co-transfected with luciferase reporter plasmid containing five copies of HREs and pRL-SV40 plasmid encoding Renilla luciferase incubated with compound under normoxia for 1 hr followed by hypoxia induction and measured after 24 hrs by HRE-dependent dual luciferase reporter gene assay
ChEMBL_1891621 (CHEMBL4393448) Inhibition of hypoxia-induced HIF1alpha transcriptional activity in human Hep3B cells after 24 hrs in hypoxic condition by HRE-dependent dual luciferase reporter gene assay
ChEMBL_1766949 (CHEMBL4202196) Inhibition of HIF-1alpha in human HRE-A549 cells pre-incubated for 1 hr before exposure to hypoxia for 24 hrs by HRE-luciferase reporter gene assay
ChEMBL_881913 (CHEMBL2212516) Inhibition of topoisomerase-1 in human U251 cells assessed as inhibition of HIF-1-mediated hypoxia-induced VEGF expression after 24 hrs by luciferase reporter gene assay
ChEMBL_881914 (CHEMBL2212517) Inhibition of topoisomerase-1 in human U251 cells assessed as inhibition of hypoxia-induced HIF-1alpha accumulation in nuclear extract after 6 to 24 hrs by immunoblot analysis
ChEMBL_1465276 (CHEMBL3406495) Inhibition of HIF1 in human HT1080 cells transfected with 5xHRE/pGL3/VEGF/E1b reporter plasmid pre-incubated for 1 hr followed by incubation under hypoxia conditions for 24 hrs by HRE-driven luciferase reporter gene assay
ChEMBL_860195 (CHEMBL2168935) Inhibition of P300 CH1 domain-mediated HIF1 transcriptional activity in human LN229-V6R cells assessed as reduction in luciferase activity incubated for 1 hr under normoxia followed by 24 hrs under hypoxia by reporter gene assay
ChEMBL_829292 (CHEMBL2060139) Inhibition of HIF1 transcriptional activity in human LN229 cells expressing VEGF-HRE-V6R and coexpressing luciferase, lac Z gene incubated for 1 hr in normoxia condition followed by 24 hrs in hypoxia condition by reporter gene assay
ChEMBL_2050089 (CHEMBL4704788) Inhibition of hypoxia-induced HIF1alpha transcriptional activity in human Hep3B cells co-transfected with luciferase reporter plasmid containing six copies of HREs and pRL-CMV vector assessed as reduction in luciferase activity incubated for 24 hrs under hypoxic condition by HRE-dependent dual luciferase reporter gene assay
ChEMBL_2102766 (CHEMBL4811162) Inhibition of hypoxia-induced HIF1 alpha transcriptional activity in human HeLa cells transfected with luciferase reporter containing of HRE assessed as reduction in luciferase activity incubated for 1 hrs under normoxic condition followed by incubation under hypoxic condition for 24 hrs by luciferase reporter gene assay
ChEMBL_2128544 (CHEMBL4837973) Inhibition of hypoxia-induced HIF1alpha transcriptional activity in human Hep3B cells co-transfected with luciferase reporter plasmid containing six copies of HREs and pRL-CMV vector assessed as reduction in luciferase activity incubated for 24 hrs under hypoxic condition by HRE-dependent dual luciferase reporter gene assay
SAR analysis for the identification of translation initiation inhibitors (PABP) Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute(SBMRI, San Diego, CA) Network: NIH Molecular Libraries Probe Production Centers Network (MLPCN) Grant Number: 1R03MH084835-01 Assay Provider: Jerry Pelletier, Ph.D, McGill University, Montreal, Canada Translation is an essential cellular process whose deregulation is associated with alterations in cell growth, cell cycle progression, and cell death responses. The initiation phase of translation is a key target for regulation when cells are exposed to various environmental cues (e.g. insulin, amino acid starvation, mitogenic stimulation, hypoxia, etc). As well, translation initiation control is usurped upon viral infection and is deregulated in many human cancers. Over-expression of certain translation factors can lead to malignant transformation and many of the components of the translational apparatus are over-expressed in human cancers. Several tumor sup
SAR analysis for the identification of translation initiation inhibitors (eIF4H) Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBIMR, San Diego, CA) Network: NIH Molecular Libraries Probe Production Centers Network (MLPCN) Grant Number: 1R03MH084835-01 Assay Provider: Jerry Pelletier, Ph.D, McGill University, Montreal, Canada Translation is an essential cellular process whose deregulation is associated with alterations in cell growth, cell cycle progression, and cell death responses. The initiation phase of translation is a key target for regulation when cells are exposed to various environmental cues (e.g. insulin, amino acid starvation, mitogenic stimulation, hypoxia, etc). As well, translation initiation control is usurped upon viral infection and is deregulated in many human cancers. Over-expression of certain translation factors can lead to malignant transformation and many of the components of the translational apparatus are over-expressed in human cancers. Several tumor su
uHTS fluorescence polarization assay for the identification of translation initiation inhibitors (PABP) Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Production Centers Network (MLPCN) Grant Number: 1R03MH084835-01 Assay Provider: Jerry Pelletier, Ph.D, McGill University, Montreal, Canada Translation is an essential cellular process whose deregulation is associated with alterations in cell growth, cell cycle progression, and cell death responses. The initiation phase of translation is a key target for regulation when cells are exposed to various environmental cues (e.g. insulin, amino acid starvation, mitogenic stimulation, hypoxia, etc). As well, translation initiation control is usurped upon viral infection and is deregulated in many human cancers. Over-expression of certain translation factors can lead to malignant transformation and many of the components of the translational apparatus are over-expressed in human cancers. Several tumor suppresso
uHTS fluorescence polarization assay for the identification of translation initiation inhibitors (eIF4H) Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Production Centers Network (MLPCN) Grant Number: 1R03MH084835-01 Assay Provider: Jerry Pelletier, Ph.D, McGill University, Montreal, Canada Translation is an essential cellular process whose deregulation is associated with alterations in cell growth, cell cycle progression, and cell death responses. The initiation phase of translation is a key target for regulation when cells are exposed to various environmental cues (e.g. insulin, amino acid starvation, mitogenic stimulation, hypoxia, etc). As well, translation initiation control is usurped upon viral infection and is deregulated in many human cancers. Over-expression of certain translation factors can lead to malignant transformation and many of the components of the translational apparatus are over-expressed in human cancers. Several tumor suppresso
Inhibitory Effect of the Compounds on PHD2 The interaction between hypoxia-inducible factor HIF-1α and VBC complex (von Hippel-Lindau protein-Elongin B-Elongin C, VBC) was detected by Fluorescence polarization (FP) method, to measure the enzyme inhibitory activity of the HIF Prolyl hydroxylases 2 (PHD2) inhibitor compounds.To a NETN (20 mM Tris.HCl, 100 mM NaCl, 1 mM EDTA, 0.5% NP-40, 1 mM PMSF) buffer containing 200 μM ascorbic acid, 20 μM α-ketoglutaric acid, 100 μM FeCl2 was added FAM-HIF (556-575) at a final concentration of 1 μM in the dark. Subsequently, the desired concentration of the test compound or the positive compound was added (the compound was replaced by the buffer in the negative control and the positive control). Finally, PHD2 was added at a final concentration of 0.5 μg/μl (PHD2 was replaced by the buffer in the negative control). They were mixed well and allowed to stand at room temperature for 30 minutes in the dark followed by 95° C. water bath for 1 minute, and then the reaction was terminated. After the temperature drops to room temperature, the sample was prepared well for use. EBC buffer (50 mM Tris.HCl, 120 mM NaCl, 0.5% NP-40) was added to the corresponding wells of a black 96-well test plate. A GST-VBC complex was added to the corresponding test wells at a final concentration of 300 nM (using the wells containing only EBC buffer as blank wells). Subsequently, the corresponding PHD2 prolyl hydroxylation reaction sample was added in the dark as a substrate with a final concentration of 100 nM. After mixing well, the lateral and longitudinal fluorescence intensity values were measured using a full-wavelength multifunctional microplate reader (TECAN infinite M1000) at an excitation wavelength of 407 nm and an emission wavelength of 518 nm.

Query String: Hypoxia