Reaction Details  Report a problem with these data
Cell Reactant:
DNA
Syringe Reactant:
vnd/NK-2 HD Mutant (H52R/T56W)
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
08/31/01
ΔG°:
-47.234±1.254 (kJ/mole)
pH:
7.4±0.01
Log10Kb:
7
Temperature:
298.15±n/a (K)
ΔHobs :
-37.62±2.508 (kJ/mole)
Ionic Strength:
50±n/a
Corrected for ΔHioniz:
yes
ΔCp :
-0.418±0.1672 (kJ/mole)
Stoich. Param.:
1
Citation
 Gonzalez, M; Weiler, S; Ferretti, JA; Ginsburg, A The vnd/NK-2 homeodomain: thermodynamics of reversible unfolding and DNA binding for wild-type and with residue replacements H52R and H52R/T56W in helix III. Biochemistry 40:4923-31 (2001) [PubMed]  Article
Cell React
Source:
purchased
Name:
DNA
Synonyms:
n/a
Type:
Duplex DNA
Mol. Mass.:
6891.48
Organism:
Synthetic
Description:
n/a
Residue:
72
Sequence:
SINGLESTRANDASEDNATGTGTCAAGTGGCTGTAGANDITSCMPLEMENTARYCTACAGCCACTTGACACA
  
Syringe React
Source:
own production
Purity:
100
Prep. Method:
overproduction in E. coli, His-tag purified, dialyzed, liophylized, resuspended and dialyzed in working buffer
Name:
vnd/NK-2 HD Mutant (H52R/T56W)
Synonyms:
HD(H52R/T56W)
Type:
n/a
Mol. Mass.:
358.43
Organism:
n/a
Description:
n/a
Residue:
3
Sequence:
NA