Reaction Details Report a problem with these data
Target
Methionine aminopeptidase
Ligand
BDBM17861
Substrate
Met-Gly-Met-Met
Meas. Tech.
EcMAP Enzyme Inhibition Assay
pH
7.5±n/a
Temperature
303.15±n/a K
IC50
2000±n/a nM
Citation
 Evdokimov, AG; Pokross, M; Walter, RL; Mekel, M; Barnett, BL; Amburgey, J; Seibel, WL; Soper, SJ; Djung, JF; Fairweather, N; Diven, C; Rastogi, V; Grinius, L; Klanke, C; Siehnel, R; Twinem, T; Andrews, R; Curnow, A Serendipitous discovery of novel bacterial methionine aminopeptidase inhibitors. Proteins 66:538-46 (2007) [PubMed]  Article 
Target
Name:
Methionine aminopeptidase
Synonyms:
EcMetAP | MAP1_ECOLI | Methionine Aminopeptidase (MAP) | Methionine aminopeptidase | Peptidase M | map
Type:
Enzyme
Mol. Mass.:
29326.96
Organism:
Escherichia coli (strain K12)
Description:
Full-length untagged EcMAP was expressed in E. coli.
Residue:
264
Sequence:
MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRICNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIKDGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAEGFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMKDGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE
  
Inhibitor
Name:
BDBM17861
Synonyms:
3,5-Pyrazolediamine Inhibitor, 10 | 3-[(E)-2-(5-amino-3-imino-3H-pyrazol-4-yl)diazen-1-yl]-2-hydroxybenzoic acid
Type:
Small organic molecule
Emp. Form.:
C10H8N6O3
Mol. Mass.:
260.2089
SMILES:
OC(=O)c1cccc(NN=c2c(=N)nnc2=N)c1O |(1.28,9.36,;2.05,8.02,;3.59,8.02,;1.28,6.69,;2.05,5.36,;1.28,4.02,;-.26,4.02,;-1.03,5.36,;-2.57,5.36,;-3.34,4.02,;-4.88,4.02,;-6.13,4.93,;-6.13,6.47,;-7.37,4.02,;-6.9,2.56,;-5.35,2.56,;-4.45,1.31,;-.26,6.69,;-1.03,8.02,)|
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
Met-Gly-Met-Met
Synonyms:
n/a
Type:
Peptide
Mol. Mass.:
468.65
Organism:
n/a
Description:
n/a
Residue:
4
Sequence:
MGMM