Reaction Details Report a problem with these data
Target
Fatty acid synthase [2202-2509]
Ligand
BDBM24978
Substrate
BDBM24568
Meas. Tech.
Fluorogenic Assay for Detection of FASTE Inhibition
pH
7.4±n/a
Temperature
310.15±n/a K
IC50
61200±n/a nM
Citation
More Info.:
Target
Name:
Fatty acid synthase [2202-2509]
Synonyms:
FAS | FASN | FAS_HUMAN | Fatty Acid Synthase
Type:
Thioesterase domain
Mol. Mass.:
33927.11
Organism:
Homo sapiens (Human)
Description:
The recombinant thioesterase domain (residues 2202-2509) of FAS was cloned and expressed in Escheria coli. The thioesterase was purified by Ni-affinity chromatography, and analyzed for activity and inhibition by Orlistat.
Residue:
308
Sequence:
CPTPKEDGLAQQQTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASRLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVR
Inhibitor
Name:
BDBM24978
Synonyms:
(3R,4R)-1-(benzyloxy)-3-hexyl-4-[(2R)-2-hydroxytridecyl]azetidin-2-one | beta-lactam derivative of orlistat, (+)-10
Type:
Small organic molecule
Emp. Form.:
C29H49NO3
Mol. Mass.:
459.7043
SMILES:
CCCCCCCCCCC[C@@H](O)C[C@@H]1[C@@H](CCCCCC)C(=O)N1OCc1ccccc1 |r|