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Target
Ribonuclease H1
Ligand
BDBM33963
Substrate
RNA/DNA hybrid
Meas. Tech.
FRET-Based RNase H Assay
pH
8±n/a
Temperature
293.15±n/a K
IC50
29000±n/a nM
Citation
 Wendeler, M; Lee, HF; Bermingham, A; Miller, JT; Chertov, O; Bona, MK; Baichoo, NS; Ehteshami, M; Beutler, J; O'Keefe, BR; Götte, M; Kvaratskhelia, M; Le Grice, S Vinylogous ureas as a novel class of inhibitors of reverse transcriptase-associated ribonuclease H activity. ACS Chem Biol 3:635-44 (2008) [PubMed]  Article 
Target
Name:
Ribonuclease H1
Synonyms:
RNASEH1 | RNH1 | RNH1_HUMAN | RNase H1 | Ribonuclease H type II
Type:
Enzyme
Mol. Mass.:
32076.45
Organism:
Homo sapiens (Human)
Description:
n/a
Residue:
286
Sequence:
MSWLLFLAHRVALAALPCRRGSRGFGMFYAVRRGRKTGVFLTWNECRAQVDRFPAARFKKFATEDEAWAFVRKSASPEVSEGHENQHGQESEAKASKRLREPLDGDGHESAEPYAKHMKPSVEPAPPVSRDTFSYMGDFVVVYTDGCCSSNGRRRPRAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALERLTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQSED
  
Inhibitor
Name:
BDBM33963
Synonyms:
vinylogous urea, NSC727448
Type:
Small organic molecule
Emp. Form.:
C12H12N2O3S
Mol. Mass.:
264.3
SMILES:
Cc1sc(NC(=O)c2ccco2)c(C(N)=O)c1C
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
RNA/DNA hybrid
Synonyms:
n/a
Type:
fluorescein-labeled RNA annealed to DNA
Mol. Mass.:
2134.40
Organism:
n/a
Description:
It is a 18-nucleotide 3-fluorescein-labeled RNA annealed to a complementary 18-nucleotide 5-Dabcyl-modified DNA.
Residue:
23
Sequence:
GACGAGCCGGGAGCFLRESCEIN