Affinity DataIC50: 1nMAssay Description:Inhibition of human EPHA4 using poly[Glu:Tyr] (4:1) as substrate preincubated for 20 mins followed by [gamma-33P]-ATP addition and measured after 120...More data for this Ligand-Target Pair
Affinity DataKd: 1.20nMAssay Description:Binding constant for EPHA4 kinase domainMore data for this Ligand-Target Pair
Affinity DataKd: 1.20nMAssay Description:Kinase inhibitors are a new class of therapeutics with a propensity to inhibit multiple targets. The biological consequences of multi-kinase activity...More data for this Ligand-Target Pair
Affinity DataKd: 1.20nMAssay Description:Binding constant for EPHA4 kinase domainMore data for this Ligand-Target Pair
Affinity DataKd: 1.90nMAssay Description:Binding constant for EPHA4 kinase domainMore data for this Ligand-Target Pair
Affinity DataIC50: 3.30nMAssay Description:Inhibition of EphA4 by [gamma33-P]ATP based assayMore data for this Ligand-Target Pair
Affinity DataIC50: 4.10nMAssay Description:Inhibition of Nano Luc-fused full length C-terminal EPHA4 (unknown origin) expressed in HEK293T cells using NanoGlo as substrate incubated for 2 hrs ...More data for this Ligand-Target Pair
Affinity DataKd: 5nMAssay Description:Binding affinity to human EPHA4More data for this Ligand-Target Pair
Affinity DataKd: 7.30nMAssay Description:Binding affinity to human EPHA4 incubated for 45 mins by Kinobead based pull down assayMore data for this Ligand-Target Pair
Affinity DataIC50: 7.60nMAssay Description:Inhibition of Nano Luc-fused full length C-terminal EPHA4 (unknown origin) expressed in HEK293T cells using NanoGlo as substrate incubated for 2 hrs ...More data for this Ligand-Target Pair
Affinity DataKd: 8.30nMAssay Description:Binding constant for EPHA4 kinase domainMore data for this Ligand-Target Pair
Affinity DataIC50: 8.5nMAssay Description:Inhibition of human EPHA4 using poly[Glu:Tyr] (4:1) as substrate by [gamma-33P]-ATP assayMore data for this Ligand-Target Pair
Affinity DataIC50: 9nMAssay Description:Inhibition of EphA4 (unknown origin) in presence of [gamma33P]ATPMore data for this Ligand-Target Pair
Affinity DataIC50: 12nMAssay Description:Antagonist activity against human EphA4 receptor assessed as inhibition of binding of alkaline phosphatase-fused ephrin-A5 to immobilized EphA4 Fc by...More data for this Ligand-Target Pair
Affinity DataIC50: 12nMAssay Description:Antagonist activity against human EphA4 receptor assessed as inhibition of binding of alkaline phosphatase-fused ephrin-A5 to immobilized EphA4 Fc by...More data for this Ligand-Target Pair
Affinity DataIC50: 12.5nMAssay Description:Inhibition of human EPHA4 using poly[Glu:Tyr] (4:1) as substrate by [gamma-33P]-ATP assayMore data for this Ligand-Target Pair
Affinity DataIC50: 17nMAssay Description:Antagonist activity against human EphA4 receptor assessed as inhibition of binding of alkaline phosphatase-fused ephrin-A5 to immobilized EphA4 Fc by...More data for this Ligand-Target Pair
Affinity DataKd: 18nMAssay Description:Binding constant for EPHA4 kinase domainMore data for this Ligand-Target Pair
Affinity DataKd: 19nMAssay Description:Binding affinity to 6His-tagged EphA4 C204A mutant (29 to 104 residues) (unknown origin) expressed in Escherichia coli Origami 2 (DE3) by isothermal ...More data for this Ligand-Target Pair
Affinity DataIC50: 22nMAssay Description:Antagonist activity against human EphA4 receptor assessed as inhibition of binding of alkaline phosphatase-fused ephrin-A5 to immobilized EphA4 Fc by...More data for this Ligand-Target Pair
Affinity DataIC50: 25nMAssay Description:Antagonist activity against human EphA4 receptor assessed as inhibition of binding of alkaline phosphatase-fused ephrin-A5 to immobilized EphA4 Fc by...More data for this Ligand-Target Pair
Affinity DataKd: 27nMAssay Description:Binding affinity to 6His-tagged EphA4 C204A mutant (29 to 104 residues) (unknown origin) expressed in Escherichia coli Origami 2 (DE3) by isothermal ...More data for this Ligand-Target Pair
Affinity DataIC50: 27nMAssay Description:Antagonist activity against human EphA4 receptor assessed as inhibition of binding of alkaline phosphatase-fused ephrin-A5 to immobilized EphA4 Fc by...More data for this Ligand-Target Pair
Affinity DataKd: 30nMAssay Description:Binding affinity to 6His-tagged EphA4 C204A mutant (29 to 104 residues) (unknown origin) expressed in Escherichia coli Origami 2 (DE3) by isothermal ...More data for this Ligand-Target Pair
Affinity DataIC50: 34nMAssay Description:Antagonist activity against human EphA4 receptor assessed as inhibition of binding of alkaline phosphatase-fused ephrin-A5 to immobilized EphA4 Fc by...More data for this Ligand-Target Pair
Affinity DataEC50: 37nMAssay Description:Inhibition of full length EPHA4 (unknown origin) expressed in HEK293T cells using NanoBRET NanoGlo substrate incubated for 2 hrs in presence of K4 tr...More data for this Ligand-Target Pair
Affinity DataEC50: 37nMAssay Description:Inhibition of full length EPHA4 (unknown origin) expressed in HEK293T cells using NanoBRET NanoGlo substrate incubated for 2 hrs in presence of K4 tr...More data for this Ligand-Target Pair
Affinity DataIC50: 38nMAssay Description:Antagonist activity against human EphA4 receptor assessed as inhibition of binding of alkaline phosphatase-fused ephrin-A5 to immobilized EphA4 Fc by...More data for this Ligand-Target Pair
Affinity DataIC50: 40nMAssay Description:Antagonist activity against human EphA4 receptor assessed as inhibition of binding of alkaline phosphatase-fused ephrin-A5 to immobilized EphA4 Fc by...More data for this Ligand-Target Pair
Affinity DataIC50: 41nMAssay Description:Antagonist activity against human EphA4 receptor assessed as inhibition of binding of alkaline phosphatase-fused ephrin-A5 to immobilized EphA4 Fc by...More data for this Ligand-Target Pair
Affinity DataKd: 45nMAssay Description:Binding affinity to 6His-tagged EphA4 C204A mutant (29 to 104 residues) (unknown origin) expressed in Escherichia coli Origami 2 (DE3) by isothermal ...More data for this Ligand-Target Pair
Affinity DataEC50: 48nMAssay Description:Inhibition of full length EPHA4 (unknown origin) expressed in HEK293T cells using NanoBRET NanoGlo substrate incubated for 2 hrs in presence of K4 tr...More data for this Ligand-Target Pair
Affinity DataIC50: 50nMAssay Description:Antagonist activity against human EphA4 receptor assessed as inhibition of binding of alkaline phosphatase-fused ephrin-A5 to immobilized EphA4 Fc by...More data for this Ligand-Target Pair
Affinity DataIC50: 54nMAssay Description:Antagonist activity against human EphA4 receptor assessed as inhibition of binding of alkaline phosphatase-fused ephrin-A5 to immobilized EphA4 Fc by...More data for this Ligand-Target Pair
Affinity DataEC50: 57nMAssay Description:Inhibition of full length EPHA4 (unknown origin) expressed in HEK293T cells using NanoBRET NanoGlo substrate incubated for 2 hrs in presence of K4 tr...More data for this Ligand-Target Pair
Affinity DataKd: 60nMAssay Description:Binding affinity to N-terminal His tagged EphA4 LBD (unknown origin) (29 to 209 residues) expressed in Rosetta-Gami B(DE3) cells by isothermal titrat...More data for this Ligand-Target Pair
Affinity DataEC50: 60nMAssay Description:Inhibition of full length EPHA4 (unknown origin) expressed in HEK293T cells using NanoBRET NanoGlo substrate incubated for 2 hrs in presence of K4 tr...More data for this Ligand-Target Pair
TargetEphrin type-A receptor 4 [29-209,M164A](Human)
Sanford Burnham Prebys Medical Discovery Institute
Sanford Burnham Prebys Medical Discovery Institute
Affinity DataKd: 62nMpH: 6.5 T: 2°CAssay Description:The purified EphA proteins (EphA4-LBD, EphA3-LBD, and EphA2-LBD) were dissolved in 50 mM potassium phosphate buffer (pH 6.5) containing 100 mM NaCl. ...More data for this Ligand-Target Pair
Affinity DataIC50: 74nMAssay Description:Inhibition of EphA4 (unknown origin)More data for this Ligand-Target Pair
Affinity DataKd: 77nMAssay Description:Binding affinity to N-terminal His tagged EphA4 LBD (unknown origin) (29 to 209 residues) expressed in Rosetta-Gami B(DE3) cells by isothermal titrat...More data for this Ligand-Target Pair
Affinity DataIC50: 83nMAssay Description:Antagonist activity against human EphA4 receptor assessed as inhibition of binding of alkaline phosphatase-fused ephrin-A5 to immobilized EphA4 Fc by...More data for this Ligand-Target Pair
Affinity DataEC50: 84nMAssay Description:Inhibition of full length EPHA4 (unknown origin) expressed in HEK293T cells using NanoBRET NanoGlo substrate incubated for 2 hrs in presence of K4 tr...More data for this Ligand-Target Pair
Affinity DataKd: 94nMAssay Description:Binding affinity to N-terminal His tagged EphA4 LBD (unknown origin) (29 to 209 residues) expressed in Rosetta-Gami B(DE3) cells by isothermal titrat...More data for this Ligand-Target Pair
Affinity DataKd: 106nMAssay Description:Binding affinity to N-terminal His tagged EphA4 LBD (unknown origin) (29 to 209 residues) expressed in Rosetta-Gami B(DE3) cells by isothermal titrat...More data for this Ligand-Target Pair
Affinity DataKd: 106nMAssay Description:Binding affinity to N-terminal His tagged EphA4 LBD (unknown origin) (29 to 209 residues) expressed in Rosetta-Gami B(DE3) cells by isothermal titrat...More data for this Ligand-Target Pair
Affinity DataKd: 113nMAssay Description:Binding affinity to N-terminal His tagged EphA4 LBD (unknown origin) (29 to 209 residues) expressed in Rosetta-Gami B(DE3) cells by isothermal titrat...More data for this Ligand-Target Pair
Affinity DataKd: 132nMAssay Description:Binding affinity to N-terminal His tagged EphA4 LBD (unknown origin) (29 to 209 residues) expressed in Rosetta-Gami B(DE3) cells by isothermal titrat...More data for this Ligand-Target Pair
Affinity DataKd: 144nMAssay Description:Binding affinity to N-terminal His tagged EphA4 LBD (unknown origin) (29 to 209 residues) expressed in Rosetta-Gami B(DE3) cells by isothermal titrat...More data for this Ligand-Target Pair
Affinity DataKd: 150nMAssay Description:Binding constant for EPHA4 kinase domainMore data for this Ligand-Target Pair


3D Structure (crystal)





































