BDBM18069 5-[(3,4,5-trimethoxyphenyl)methyl]pyrimidine-2,4-diamine::CHEMBL22::TMP::Trimethoprim::Trimethoprim (TMP)::US10870625, Compound TMP
SMILES COc1cc(Cc2cnc(N)nc2N)cc(OC)c1OC
InChI Key InChIKey=IEDVJHCEMCRBQM-UHFFFAOYSA-N
Activity Spreadsheet -- Enzyme Inhibition Constant Data from BindingDB
Found 313 hits for monomerid = 18069
Affinity DataKi: 0.220nMAssay Description:Inhibition of recombinant Escherichia coli DHFR expressed in Escherichia coli BL21(DE3) cells assessed as reduction in NADPH oxidation using dihydrof...More data for this Ligand-Target Pair
Affinity DataKi: 1.20nMAssay Description:Inhibition of Staphylococcus aureus DHFR using dihydrofolate as substrate preincubated for 10 mins followed by substrate addition by spectrophotometr...More data for this Ligand-Target Pair
Affinity DataKi: 1.24nMAssay Description:Inhibition of Staphylococcus aureus DHFR assessed as oxidation of NADPH using dihydrofolate as substrate pre-incubated for 10 mins before substrate a...More data for this Ligand-Target Pair
Affinity DataKi: 1.30nMAssay Description:In vitro inhibition of rat liver dihydrofolate reductase.More data for this Ligand-Target Pair
Affinity DataKi: 1.30nMAssay Description:Inhibition of Rap1A-mediated geranylgeranylation expressed in mouse NIH3T3 cellsMore data for this Ligand-Target Pair
Affinity DataKi: 1.30nMAssay Description:Binding affinity against Dihydrofolate reductase of Escherichia coliMore data for this Ligand-Target Pair
Affinity DataKi: 1.30nMAssay Description:Apparent inhibitory (log 1/Ki) activity against Escherichia coli dihydrofolate reductaseMore data for this Ligand-Target Pair
Affinity DataKi: 1.30nMAssay Description:Apparent binding affinity against Dihydrofolate reductase in Escherichia coliChecked by AuthorMore data for this Ligand-Target Pair
Affinity DataKi: 1.30nMAssay Description:Compound is evaluated for the inhibition of dihydrofolate reductase from Escherichia coliMore data for this Ligand-Target Pair
Affinity DataKi: 1.30nMAssay Description:Binding affinity for E. coli Dihydrofolate reductaseMore data for this Ligand-Target Pair
Affinity DataKi: 1.35nMAssay Description:Inhibition constant against binding of Escherichia coli dihydrofolate reductaseMore data for this Ligand-Target Pair
Affinity DataKi: 3nMAssay Description:Inhibition assay using Esherichia coli dihydrofolate reductase (DHFR). More data for this Ligand-Target Pair
Affinity DataKi: 3.40nMAssay Description:Inhibition of TMP/methicillin-resistant Staphylococcus aureus wild type DHFR assessed as oxidation of NADPH pre-incubated for 5 mins followed by dihy...More data for this Ligand-Target Pair
Affinity DataKi: 3.40nMAssay Description:Inhibition of recombinant Staphylococcus aureus DHFR expressed in Escherichia coli BL21(DE3) cells assessed as reduction in NADPH oxidation using dih...More data for this Ligand-Target Pair
Affinity DataKi: 3.80nMAssay Description:Inhibition of Escherichia coli Dihydrofolate reductase in presence of 100 uM Dihydrofolate reductaseMore data for this Ligand-Target Pair
Affinity DataKi: 4.40nMAssay Description:Inhibition of Staphylococcus aureus wild type recombinant DHFR by MTS assayMore data for this Ligand-Target Pair
Affinity DataKi: 5.10nMAssay Description:Antibacterial activity against Staphylococcus aureusMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Janssen Research and Development LLC
Curated by ChEMBL
Janssen Research and Development LLC
Curated by ChEMBL
Affinity DataKi: 6nMAssay Description:Binding affinity to DHFR (unknown origin) by NMR analysisMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Salmonella enterica subsp. enterica serovar Typhi)
Pomona College
Curated by ChEMBL
Pomona College
Curated by ChEMBL
Affinity DataKi: 8.30nMAssay Description:Inhibitory activity against Escherichia coli dihydrofolate reductaseMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Janssen Research and Development LLC
Curated by ChEMBL
Janssen Research and Development LLC
Curated by ChEMBL
Affinity DataKi: 10nMAssay Description:Inhibition of recombinant Dihydrofolate reductase from humans.More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Janssen Research and Development LLC
Curated by ChEMBL
Janssen Research and Development LLC
Curated by ChEMBL
Affinity DataKi: 10nMAssay Description:Inhibition of human DHFR assessed by NADPH oxidation measured by spectrophotometric methodMore data for this Ligand-Target Pair
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Plasmodium falciparum (isolate K1 / Thailand))
National Center for Genetic Engineering and Biotechnology at Thailand
Curated by ChEMBL
National Center for Genetic Engineering and Biotechnology at Thailand
Curated by ChEMBL
Affinity DataKi: 10.3nMAssay Description:Inhibitory activity against wild-type dihydrofolate reductase (S108N DHFR)More data for this Ligand-Target Pair
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Plasmodium falciparum (isolate K1 / Thailand))
National Center for Genetic Engineering and Biotechnology at Thailand
Curated by ChEMBL
National Center for Genetic Engineering and Biotechnology at Thailand
Curated by ChEMBL
Affinity DataKi: 10.3nMAssay Description:Inhibition of the wild-type dihydrofolate reductase (DHFR)More data for this Ligand-Target Pair
Affinity DataKi: 40nM ΔG°: -10.3kcal/mole IC50: 600nMpH: 7.0 T: 2°CAssay Description:Activity was measured as a change in absorbance over time at a wavelength of 340 nm (A340), so as to monitor the disappearance of NADPH. After incuba...More data for this Ligand-Target Pair
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Leishmania major)
COMSATS University Islamabad
Curated by ChEMBL
COMSATS University Islamabad
Curated by ChEMBL
Affinity DataKi: 120nMAssay Description:Inhibition of Leishmania major DHFR assessed by NADPH oxidation measured by spectrophotometric methodMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Janssen Research and Development LLC
Curated by ChEMBL
Janssen Research and Development LLC
Curated by ChEMBL
Affinity DataKi: 120nMAssay Description:Inhibition of recombinant Dihydrofolate reductase from Leishmania major.More data for this Ligand-Target Pair
Affinity DataKi: 132nMAssay Description:Inhibitory activity against Lactobacillus casei dihydrofolate reductaseMore data for this Ligand-Target Pair
Affinity DataKi: 132nMAssay Description:Inhibitory activity against dihydrofolate reductase (DHFR) from Lactobacillus casei (expressed as log 1/Kiapp)More data for this Ligand-Target Pair
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Plasmodium falciparum (isolate K1 / Thailand))
National Center for Genetic Engineering and Biotechnology at Thailand
Curated by ChEMBL
National Center for Genetic Engineering and Biotechnology at Thailand
Curated by ChEMBL
Affinity DataKi: 132nMAssay Description:Inhibition of the S108N mutant of dihydrofolate reductase (DHFR)More data for this Ligand-Target Pair
Affinity DataKi: 151nMAssay Description:Inhibition of dihydrofolate reductase (DHFR) from Pneumocystis carinii.More data for this Ligand-Target Pair
Affinity DataKi: 152nMAssay Description:Binding affinity of the compound was reported with purified recombinant P. carnii Dihydrofolate reductaseMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Janssen Research and Development LLC
Curated by ChEMBL
Janssen Research and Development LLC
Curated by ChEMBL
Affinity DataKi: 195nMAssay Description:Inhibition of human dihydrofolate reductase (DHFR) enzymeMore data for this Ligand-Target Pair
Affinity DataKi: 240nM ΔG°: -9.18kcal/mole IC50: 3.00E+3nMpH: 7.0 T: 2°CAssay Description:Activity was measured as a change in absorbance over time at a wavelength of 340 nm (A340), so as to monitor the disappearance of NADPH. After incuba...More data for this Ligand-Target Pair
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Plasmodium falciparum (isolate K1 / Thailand))
National Center for Genetic Engineering and Biotechnology at Thailand
Curated by ChEMBL
National Center for Genetic Engineering and Biotechnology at Thailand
Curated by ChEMBL
Affinity DataKi: 242nMAssay Description:Inhibition of the C59R+S108N mutant of dihydrofolate reductase (DHFR)More data for this Ligand-Target Pair
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Plasmodium falciparum (isolate K1 / Thailand))
National Center for Genetic Engineering and Biotechnology at Thailand
Curated by ChEMBL
National Center for Genetic Engineering and Biotechnology at Thailand
Curated by ChEMBL
Affinity DataKi: 242nMAssay Description:Inhibitory activity against double mutant dihydrofolate reductase (C59R+S108N DHFR)More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Janssen Research and Development LLC
Curated by ChEMBL
Janssen Research and Development LLC
Curated by ChEMBL
Affinity DataKi: 1.00E+3nMAssay Description:Inhibition of recombinant Dihydrofolate reductase from Trypanosoma cruzi.More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Janssen Research and Development LLC
Curated by ChEMBL
Janssen Research and Development LLC
Curated by ChEMBL
Affinity DataKi: 1.38E+3nMAssay Description:Inhibition of recombinant Dihydrofolate reductase from humans.More data for this Ligand-Target Pair
Affinity DataKi: 3.09E+3nMpH: 7.2Assay Description:Inhibitory activity against bovine liver dihydrofolate reductase at pH 7.2.More data for this Ligand-Target Pair
Affinity DataKi: 3.09E+3nMpH: 7.2Assay Description:Inhibition of Wnt2/beta-catenin signaling in human A549 cells assessed as inhibition of cell proliferation after 48 hrs by MTT assayMore data for this Ligand-Target Pair
Affinity DataKi: 3.09E+3nMAssay Description:Inhibition of dihydrofolate reductase from bovine liverMore data for this Ligand-Target Pair
Affinity DataKi: 5.50E+3nM ΔG°: -7.29kcal/mole IC50: 4.50E+4nMpH: 7.0 T: 2°CAssay Description:Activity was measured as a change in absorbance over time at a wavelength of 340 nm (A340), so as to monitor the disappearance of NADPH. After incuba...More data for this Ligand-Target Pair
Affinity DataKi: 5.60E+3nM ΔG°: -7.28kcal/mole IC50: 4.20E+4nMpH: 7.0 T: 2°CAssay Description:Activity was measured as a change in absorbance over time at a wavelength of 340 nm (A340), so as to monitor the disappearance of NADPH. After incuba...More data for this Ligand-Target Pair
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Plasmodium falciparum (isolate K1 / Thailand))
National Center for Genetic Engineering and Biotechnology at Thailand
Curated by ChEMBL
National Center for Genetic Engineering and Biotechnology at Thailand
Curated by ChEMBL
Affinity DataKi: 5.69E+3nMAssay Description:Inhibitory activity against triple mutant dihydrofolate reductase (C59R S108 NI164L DHFR)More data for this Ligand-Target Pair
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Plasmodium falciparum (isolate K1 / Thailand))
National Center for Genetic Engineering and Biotechnology at Thailand
Curated by ChEMBL
National Center for Genetic Engineering and Biotechnology at Thailand
Curated by ChEMBL
Affinity DataKi: 6.66E+3nMAssay Description:Inhibitory activity against quadruple mutant dihydrofolate reductase (N51I C59R S108N I164L DHFR)More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Janssen Research and Development LLC
Curated by ChEMBL
Janssen Research and Development LLC
Curated by ChEMBL
Affinity DataKi: 1.91E+4nMAssay Description:Inhibition of human DHFR using dihydrofolate as substrate preincubated for 10 mins followed by substrate addition by spectrophotometric analysis in p...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Janssen Research and Development LLC
Curated by ChEMBL
Janssen Research and Development LLC
Curated by ChEMBL
Affinity DataKi: 1.91E+4nMAssay Description:Inhibition of human DHFR assessed as oxidation of NADPH using dihydrofolate as substrate pre-incubated for 10 mins before substrate addition by spect...More data for this Ligand-Target Pair
Affinity DataKi: 2.41E+4nMAssay Description:Inhibition of wild type Bacillus anthracis recombinant DHFRMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Janssen Research and Development LLC
Curated by ChEMBL
Janssen Research and Development LLC
Curated by ChEMBL
Affinity DataKi: 4.58E+4nMAssay Description:Inhibition of recombinant human DHFR assessed as reduction in NADPH oxidation using dihydrofolate as substrate preincubated for 5 mins followed by su...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Janssen Research and Development LLC
Curated by ChEMBL
Janssen Research and Development LLC
Curated by ChEMBL
Affinity DataKi: 4.80E+4nMAssay Description:Inhibition of human DHFRMore data for this Ligand-Target Pair
Affinity DataKi: 1.05E+5nMpH: 7.2Assay Description:Inhibitory activity against chicken dihydrofolate reductase at pH 7.2.More data for this Ligand-Target Pair