Affinity DataIC50: 1.20nMAssay Description:The kinase activity was determined by incubation of enzyme and its substrate, and test compound, in the presence ATP/[gamma-33P] ATP. After incubatio...More data for this Ligand-Target Pair
Affinity DataIC50: 2nMpH: 7.2 T: 2°CAssay Description:In this assay, a fluorescent phosphopeptide tracer and the nonfluorescent phosphopeptides generated during a PKA reaction compete for binding to an a...More data for this Ligand-Target Pair
Affinity DataIC50: 4nMpH: 7.5 T: 2°CAssay Description:An ELISA-based assay has been developed for the serine/threonine kinase, PKB-alpha. The assay utilizes an N-terminally biotinylated substrate peptide...More data for this Ligand-Target Pair
Affinity DataIC50: 5nMpH: 7.5 T: 2°CAssay Description:An ELISA-based assay has been developed for the serine/threonine kinase, PKB-alpha. The assay utilizes an N-terminally biotinylated substrate peptide...More data for this Ligand-Target Pair
Affinity DataIC50: 5nMpH: 7.2 T: 2°CAssay Description:In this assay, a fluorescent phosphopeptide tracer and the nonfluorescent phosphopeptides generated during a PKA reaction compete for binding to an a...More data for this Ligand-Target Pair
Affinity DataIC50: 20nMpH: 7.5 T: 2°CAssay Description:An ELISA-based assay has been developed for the serine/threonine kinase, PKB-alpha. The assay utilizes an N-terminally biotinylated substrate peptide...More data for this Ligand-Target Pair
Affinity DataIC50: 20nMpH: 7.5 T: 2°CAssay Description:An ELISA-based assay has been developed for the serine/threonine kinase, PKB-alpha. The assay utilizes an N-terminally biotinylated substrate peptide...More data for this Ligand-Target Pair
Affinity DataIC50: 23nMpH: 7.5 T: 2°CAssay Description:An ELISA-based assay has been developed for the serine/threonine kinase, PKB-alpha. The assay utilizes an N-terminally biotinylated substrate peptide...More data for this Ligand-Target Pair
Affinity DataIC50: 30nMpH: 7.2 T: 2°CAssay Description:In this assay, a fluorescent phosphopeptide tracer and the nonfluorescent phosphopeptides generated during a PKA reaction compete for binding to an a...More data for this Ligand-Target Pair
Affinity DataIC50: 39nMpH: 7.2 T: 2°CAssay Description:In this assay, a fluorescent phosphopeptide tracer and the nonfluorescent phosphopeptides generated during a PKA reaction compete for binding to an a...More data for this Ligand-Target Pair
Affinity DataIC50: 40nMpH: 7.5 T: 2°CAssay Description:An ELISA-based assay has been developed for the serine/threonine kinase, PKB-alpha. The assay utilizes an N-terminally biotinylated substrate peptide...More data for this Ligand-Target Pair
Affinity DataIC50: 42nMAssay Description:Inhibition of human recombinant HDAC6 assessed as fluorescence using fluorogenic substrate ZMAL incubated for 90 min by microplate readerMore data for this Ligand-Target Pair
Affinity DataIC50: <45nMpH: 6.8 T: 2°CAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
TargetcAMP-dependent protein kinase catalytic subunit alpha [V123M](Bos taurus (bovine))
German Cancer Research Center
German Cancer Research Center
Affinity DataIC50: <45nMpH: 6.8 T: 2°CAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
Affinity DataIC50: <45nMAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
TargetcAMP-dependent protein kinase catalytic subunit alpha [V123M,Q181K,T183A](Bos taurus (bovine))
German Cancer Research Center
German Cancer Research Center
Affinity DataIC50: <45nMAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
TargetcAMP-dependent protein kinase catalytic subunit alpha [L49I,Q181K,T183A](Bos taurus (bovine))
German Cancer Research Center
German Cancer Research Center
Affinity DataIC50: <45nMAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
Affinity DataIC50: 45nMAssay Description:Inhibition of human recombinant HDAC6 assessed as fluorescence using fluorogenic substrate ZMAL incubated for 90 min by microplate readerMore data for this Ligand-Target Pair
TargetcAMP-dependent protein kinase catalytic subunit alpha [Q181K,T183A](Bos taurus (bovine))
German Cancer Research Center
German Cancer Research Center
Affinity DataIC50: <45nMAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
TargetcAMP-dependent protein kinase catalytic subunit alpha [Q181K,T183A](Bos taurus (bovine))
German Cancer Research Center
German Cancer Research Center
Affinity DataIC50: <45nMAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
TargetcAMP-dependent protein kinase catalytic subunit alpha [E127D](Bos taurus (bovine))
German Cancer Research Center
German Cancer Research Center
Affinity DataIC50: <45nMAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
TargetcAMP-dependent protein kinase catalytic subunit alpha [L49I,V123M,E127D,Q181K,T183A](Bos taurus (bovine))
German Cancer Research Center
German Cancer Research Center
Affinity DataIC50: <45nMAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
TargetcAMP-dependent protein kinase catalytic subunit alpha [T183A](Bos taurus (bovine))
German Cancer Research Center
German Cancer Research Center
Affinity DataIC50: <45nMAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
TargetcAMP-dependent protein kinase catalytic subunit alpha [T183A](Bos taurus (bovine))
German Cancer Research Center
German Cancer Research Center
Affinity DataIC50: <45nMAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
Affinity DataIC50: 46nMAssay Description:Inhibition of human recombinant HDAC6 assessed as fluorescence using fluorogenic substrate ZMAL incubated for 90 min by microplate readerMore data for this Ligand-Target Pair
TargetcAMP-dependent protein kinase catalytic subunit alpha [L49I](Bos taurus (bovine))
German Cancer Research Center
German Cancer Research Center
Affinity DataIC50: 47nMpH: 6.8 T: 2°CAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
Affinity DataIC50: 51nMAssay Description:Inhibition of human recombinant HDAC6 assessed as fluorescence using fluorogenic substrate ZMAL incubated for 90 min by microplate readerMore data for this Ligand-Target Pair
TargetcAMP-dependent protein kinase catalytic subunit alpha [L49I,V123M,Q181K,T183A](Bos taurus (bovine))
German Cancer Research Center
German Cancer Research Center
Affinity DataIC50: 51nMAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
Affinity DataIC50: 56nMpH: 6.8 T: 2°CAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
TargetcAMP-dependent protein kinase catalytic subunit alpha [L49I,Q181K,T183A](Bos taurus (bovine))
German Cancer Research Center
German Cancer Research Center
Affinity DataIC50: 58nMAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
Affinity DataIC50: 64nMAssay Description:Inhibition of human HDAC3 assessed as target activity using fluorogenic substrate ZMAL for 90 min by microplate reader at 355nmMore data for this Ligand-Target Pair
Affinity DataIC50: 67nMAssay Description:Inhibition of human recombinant HDAC6 assessed as fluorescence using fluorogenic substrate ZMAL incubated for 90 min by microplate readerMore data for this Ligand-Target Pair
TargetcAMP-dependent protein kinase catalytic subunit alpha [V123M,Q181K,T183A](Bos taurus (bovine))
German Cancer Research Center
German Cancer Research Center
Affinity DataIC50: 68nMAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
Affinity DataIC50: 73nMAssay Description:Inhibition of human recombinant HDAC6 assessed as fluorescence using fluorogenic substrate ZMAL incubated for 90 min by microplate readerMore data for this Ligand-Target Pair
Affinity DataIC50: 80nMAssay Description:Inhibition of human recombinant HDAC6 assessed as fluorescence using fluorogenic substrate ZMAL incubated for 90 min by microplate readerMore data for this Ligand-Target Pair
TargetcAMP-dependent protein kinase catalytic subunit alpha [L49I,V123M,Q181K,T183A](Bos taurus (bovine))
German Cancer Research Center
German Cancer Research Center
Affinity DataIC50: 89nMAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
Affinity DataIC50: 93nMAssay Description:Inhibition of human recombinant HDAC1 assessed as fluorescence using fluorogenic substrate ZMAL incubated for 90 min by microplate readerMore data for this Ligand-Target Pair
Affinity DataIC50: 97nMAssay Description:Inhibition of human recombinant HDAC1 assessed as fluorescence using fluorogenic substrate ZMAL incubated for 90 min by microplate readerMore data for this Ligand-Target Pair
Affinity DataIC50: 108nMAssay Description:Inhibition of human HDAC3 assessed as target activity using fluorogenic substrate ZMAL for 90 min by microplate reader at 355nmMore data for this Ligand-Target Pair
TargetcAMP-dependent protein kinase catalytic subunit alpha [Q181K,T183A](Bos taurus (bovine))
German Cancer Research Center
German Cancer Research Center
Affinity DataIC50: 109nMAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
TargetcAMP-dependent protein kinase catalytic subunit alpha [T183A](Bos taurus (bovine))
German Cancer Research Center
German Cancer Research Center
Affinity DataIC50: 109nMAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
Affinity DataIC50: 119nMAssay Description:Inhibition of human HDAC3 assessed as target activity using fluorogenic substrate ZMAL for 90 min by microplate reader at 355nmMore data for this Ligand-Target Pair
Affinity DataIC50: 120nMAssay Description:Inhibition of human recombinant HDAC1 assessed as fluorescence using fluorogenic substrate ZMAL incubated for 90 min by microplate readerMore data for this Ligand-Target Pair
Affinity DataIC50: 125nMAssay Description:Inhibition of human recombinant HDAC1 assessed as fluorescence using fluorogenic substrate ZMAL incubated for 90 min by microplate readerMore data for this Ligand-Target Pair
Affinity DataIC50: 142nMAssay Description:Inhibition of human recombinant HDAC1 assessed as fluorescence using fluorogenic substrate ZMAL incubated for 90 min by microplate readerMore data for this Ligand-Target Pair
TargetcAMP-dependent protein kinase catalytic subunit alpha [L49I,V123M,E127D,Q181K,T183A](Bos taurus (bovine))
German Cancer Research Center
German Cancer Research Center
Affinity DataIC50: 149nMAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
Affinity DataIC50: 149nMAssay Description:Inhibition of human HDAC3 assessed as target activity using fluorogenic substrate ZMAL for 90 min by microplate reader at 355nmMore data for this Ligand-Target Pair
Affinity DataIC50: 160nMpH: 7.5 T: 2°CAssay Description:An ELISA-based assay has been developed for the serine/threonine kinase, PKB-alpha. The assay utilizes an N-terminally biotinylated substrate peptide...More data for this Ligand-Target Pair
Affinity DataIC50: 163nMAssay Description:Inhibition of human recombinant HDAC1 assessed as fluorescence using fluorogenic substrate ZMAL incubated for 90 min by microplate readerMore data for this Ligand-Target Pair
Affinity DataIC50: 168nMAssay Description:Inhibition of human HDAC2 assessed as target activity using fluorogenic substrate ZMAL for 90 min by microplate reader at 355nmMore data for this Ligand-Target Pair