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Target
Histone-lysine N-methyltransferase EZH2/Polycomb protein EED [40-441]/Polycomb protein SUZ12 [559-739]
Ligand
BDBM225230
Substrate
n/a
Meas. Tech.
Isothermal Titration Calorimetry (ITC)
pH
8±0
Temperature
1663.9±0 K
Kd
114±0.0 nM
Citation
 Qi, W; Zhao, K; Gu, J; Huang, Y; Wang, Y; Zhang, H; Zhang, M; Zhang, J; Yu, Z; Li, L; Teng, L; Chuai, S; Zhang, C; Zhao, M; Chan, H; Chen, Z; Fang, D; Fei, Q; Feng, L; Feng, L; Gao, Y; Ge, H; Ge, X; Li, G; Lingel, A; Lin, Y; Liu, Y; Luo, F; Shi, M; Wang, L; Wang, Z; Yu, Y; Zeng, J; Zeng, C; Zhang, L; Zhang, Q; Zhou, S; Oyang, C; Atadja, P; Li, E An allosteric PRC2 inhibitor targeting the H3K27me3 binding pocket of EED. Nat Chem Biol 13:381-388 (2017) [PubMed]  Article 
Target
Name:
Histone-lysine N-methyltransferase EZH2/Polycomb protein EED [40-441]/Polycomb protein SUZ12 [559-739]
Synonyms:
Polycomb repressive complex 2 (PRC2)
Type:
Protein
Mol. Mass.:
n/a
Description:
Human three-member PRC2 complex (EZH2(1-751 aa)/EED(40-441 aa)/SUZ12(559-739 aa))
Components:
This complex has 3 components.
Component 1
Name:
Histone-lysine N-methyltransferase EZH2
Synonyms:
Enhancer of zeste homolog 2 (EZH2)
Type:
Protein
Mol. Mass.:
86024.60
Organism:
Homo sapiens (Human)
Description:
Q15910-2
Residue:
751
Sequence:
MGQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFSSNRQKILERTEILNQEWKQRRIQPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKTLNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEKYKELTEQQLPGALPPECTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHRKCNYSFHATPNTYKRKNTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKTPPKRPGGRRRGRLPNNSSRPSTPTINVLESKDTDSDREAGTETGGENNDKEEEEKKDETSSSSEANSRCQTPIKMKPNIEPPENVEWSGAEASMFRVLIGTYYDNFCAIARLIGTKTCRQVYEFRVKESSIIAPAPAEDVDTPPRKKKRKHRLWAAHCRKIQLKKDGSSNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCGAADHWDSKNVSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREMEIP
  
Component 2
Name:
Polycomb protein EED [40-441]
Synonyms:
EED | EED_HUMAN | Polycomb protein EED (EED)
Type:
Protein
Mol. Mass.:
46101.42
Organism:
Homo sapiens (Human)
Description:
Human polycomb protein EED (40-441 aa)
Residue:
402
Sequence:
DAVSIESGTNTERPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCGMDHSLKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWLGDLILSKSCENAIVCWKPGKMEDDIDKIKPSESNVTILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAKCTTLTHHKCGAAIRQTSFSRDSSILIAVCDDASIWRWDRLR
  
Component 3
Name:
Polycomb protein SUZ12 [559-739]
Synonyms:
CHET9 | JJAZ1 | KIAA0160 | Polycomb protein SUZ12 (SUZ12) | SUZ12 | SUZ12_HUMAN
Type:
Protein
Mol. Mass.:
20870.88
Organism:
Homo sapiens (Human)
Description:
Human polycomb protein SUZ12 (559-739 aa)
Residue:
181
Sequence:
SGHNRLYFHSDTCLPLRPQEMEVDSEDEKDPEWLREKTITQIEEFSDVNEGEKEVMKLWNLHVMKHGFIADNQMNHACMLFVENYGQKIIKKNLCRNFMLHLVSMHDFNLISIMSIDKAVTKLREMQQKLEKGESASPANEEITEEQNGTANGFSEINSKEKALETDSVSGVSKQSKKQKL
  
Inhibitor
Name:
BDBM225230
Synonyms:
EED226 | US11013745, Compound EED226
Type:
Small organic molecule
Emp. Form.:
C17H15N5O3S
Mol. Mass.:
369.398
SMILES:
CS(=O)(=O)c1ccc(cc1)-c1cnc(NCc2ccco2)n2cnnc12
Structure:
Search PDB for entries with ligand similarity: