Affinity DataKd: 1.91E+3nMAssay Description:Tryptophan fluorescence measurements were performed with purified FdTSPO1 protein and ligand essentially as described by Li et al.; 2.5 μM prote...More data for this Ligand-Target Pair
Affinity DataKd: 720nMpH: 6.8 T: 2°CAssay Description:All UV-visible spectra were measured on a Varian Cary 50 spectrometer with temperature control from a Peltier cooler. The holo-enzyme was titrated in...More data for this Ligand-Target Pair
Affinity DataKd: 1.68E+3nMpH: 6.8Assay Description:Titrations using fluorescence spectroscopy were carried out on a Jobin Yvon Horiba FluoroMax-3 fluorimeter in scanning mode. Apo-HemQ (5 μM) was...More data for this Ligand-Target Pair
TargetDimer of Prostaglandin G/H synthase 2(Homo sapiens (Human))
University Of Michigan Medical School
University Of Michigan Medical School
Affinity DataKd: 130nMpH: 8.0Assay Description:POX reactions were conducted in 2 ml of filtered and degassed 50 mM Tris-HCl, pH 8.0, containing 50 mM NaCl, 0.1 mM H2O2, 4.5 mM guaiacol, and the in...More data for this Ligand-Target Pair
Affinity DataKd: 160nMpH: 8.0Assay Description:POX reactions were conducted in 2 ml of filtered and degassed 50 mM Tris-HCl, pH 8.0, containing 50 mM NaCl, 0.1 mM H2O2, 4.5 mM guaiacol, and the in...More data for this Ligand-Target Pair
TargetProstaglandin G/H synthase 2 [S530A](Homo sapiens (Human))
University Of Michigan Medical School
University Of Michigan Medical School
Affinity DataKd: 240nMpH: 8.0Assay Description:POX reactions were conducted in 2 ml of filtered and degassed 50 mM Tris-HCl, pH 8.0, containing 50 mM NaCl, 0.1 mM H2O2, 4.5 mM guaiacol, and the in...More data for this Ligand-Target Pair
TargetProstaglandin G/H synthase 2 [Y385F](Homo sapiens (Human))
University Of Michigan Medical School
University Of Michigan Medical School
Affinity DataKd: 370nMpH: 8.0Assay Description:POX reactions were conducted in 2 ml of filtered and degassed 50 mM Tris-HCl, pH 8.0, containing 50 mM NaCl, 0.1 mM H2O2, 4.5 mM guaiacol, and the in...More data for this Ligand-Target Pair
TargetProstaglandin G/H synthase 2 [R120A,Y385F](Homo sapiens (Human))
University Of Michigan Medical School
University Of Michigan Medical School
Affinity DataKd: 620nMpH: 8.0Assay Description:POX reactions were conducted in 2 ml of filtered and degassed 50 mM Tris-HCl, pH 8.0, containing 50 mM NaCl, 0.1 mM H2O2, 4.5 mM guaiacol, and the in...More data for this Ligand-Target Pair
TargetDimer of Prostaglandin G/H synthase 2 [R120Q](Homo sapiens (Human))
University Of Michigan Medical School
University Of Michigan Medical School
Affinity DataKd: 220nMpH: 8.0Assay Description:POX reactions were conducted in 2 ml of filtered and degassed 50 mM Tris-HCl, pH 8.0, containing 50 mM NaCl, 0.1 mM H2O2, 4.5 mM guaiacol, and the in...More data for this Ligand-Target Pair
TargetDimer of Prostaglandin G/H synthase 2 [R120A](Homo sapiens (Human))
University Of Michigan Medical School
University Of Michigan Medical School
Affinity DataKd: 750nMpH: 8.0Assay Description:POX reactions were conducted in 2 ml of filtered and degassed 50 mM Tris-HCl, pH 8.0, containing 50 mM NaCl, 0.1 mM H2O2, 4.5 mM guaiacol, and the in...More data for this Ligand-Target Pair
TargetDimer of Prostaglandin G/H synthase 2 [S530A](Homo sapiens (Human))
University Of Michigan Medical School
University Of Michigan Medical School
Affinity DataKd: 190nMpH: 8.0Assay Description:POX reactions were conducted in 2 ml of filtered and degassed 50 mM Tris-HCl, pH 8.0, containing 50 mM NaCl, 0.1 mM H2O2, 4.5 mM guaiacol, and the in...More data for this Ligand-Target Pair
TargetDimer of Prostaglandin G/H synthase 2 [Y385F](Homo sapiens (Human))
University Of Michigan Medical School
University Of Michigan Medical School
Affinity DataKd: 1.60E+3nMpH: 8.0Assay Description:POX reactions were conducted in 2 ml of filtered and degassed 50 mM Tris-HCl, pH 8.0, containing 50 mM NaCl, 0.1 mM H2O2, 4.5 mM guaiacol, and the in...More data for this Ligand-Target Pair