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Activity Spreadsheet -- Enzyme Inhibition Constant Data from BindingDB (change energy unit to kcal/mol)

Found 356 hits in this display   

TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataKi:  0nMAssay Description:Inhibitory activity against dihydrofolate reductase (DHFR) of L-1210 cellsMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  0.000340nMAssay Description:Inhibition of human DHFR by spectrophotometric analysisMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  0.00100nMAssay Description:Inhibition of recombinant Escherichia coli DHFRMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  0.00120nMAssay Description:Inhibitory activity against Wild-type human DHFRMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  0.00300nMAssay Description:Inhibition of recombinant human DHFR assessed as reduction in NADPH oxidation using dihydrofolate as substrate by fluorescence spectrophotometric ana...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  0.00320nMAssay Description:Tested for inhibition of dihydrofolate reductase enzymeMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  0.00400nMAssay Description:Compound was evaluated for binding affinity against Dihydrofolate reductase of L1210 cellsMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  0.00400nMAssay Description:Inhibition constant of compound against L. casei Dihydrofolate reductaseMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  0.00430nMAssay Description:Inhibition of Dihydrofolate reductase in mouse L1210 cellsMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  0.00480nMAssay Description:Compound was evaluated in vitro for the inhibition of dihydrofolate reductase (DHFR) in L1210 cellsMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  0.00482nMAssay Description:Inhibitory activity of Dihydrofolate reductase in L1210 cellsMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  0.00500nMAssay Description:Compound was evaluated for the inhibition of Dihydrofolate reductase at concentration ranged from 0.15-0.50 uMMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  0.00528nMAssay Description:The compound was tested for its inhibitory activity against Dihydrofolate reductase derived from L1210 cells.More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  0.00545nMAssay Description:Evaluated for inhibition of dihydrofolate reductase (DHFR) isolated from L1210 cellsMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  0.00548nMAssay Description:Compound was evaluated for inhibitory effect on dihydrofolate reductase (DHFR) from L1210 cells at Inhibitory constant (n=3)More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  0.00581nMAssay Description:In vitro inhibitory activity against Dihydrofolate reductase in L1210 cell in miceMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  0.00600nMAssay Description:Inhibitory activity of Dihydrofolate reductase derived from L1210 cell line.More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  0.00600nMAssay Description:Inhibition of the dihydrofolate reductase enzyme(DHFR) derived from L1210 murine leukemia cells.More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  0.00600nMAssay Description:In vitro inhibitory activity against Dihydrofolate reductase in L1210 cell in miceMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  0.00600nMAssay Description:Inhibition of Dihydrofolate reductase (DHFR) enzyme derived from L1210 cells expressed as Ki (pM)More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  0.00600nMAssay Description:In vitro inhibitory activity against L1210 dihydrofolate reductase in rodent neoplastic cellsMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Pneumocystis carinii)
Indiana University School Of Medicine

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  0.00800nMAssay Description:Binding affinity of the compound was reported with purified recombinant P. carnii Dihydrofolate reductaseMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataKi:  0.0100nMAssay Description:Inhibition of DHFR (unknown origin)More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  0.0210nMAssay Description:Binding affinity against Dihydrofolate reductase of Escherichia coliMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  0.0380nMAssay Description:Inhibitory activity against Trypanosoma cruzi dihydrofolate reductaseMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  0.110nMAssay Description:Inhibitory activity against Leu22-Phe mutant human Dihydrofolate reductaseMore data for this Ligand-Target Pair
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  0.129nMAssay Description:Inhibition of dihydrofolate reductase (DHFR) from Leishmania majorChecked by AuthorMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  0.179nMAssay Description:Inhibitory activity against dihydrofolate reductase in humansMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Staphylococcus aureus)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  1nMAssay Description:Inhibition of Staphylococcus aureus DHFRMore data for this Ligand-Target Pair
TargetPteridine reductase 1(Leishmania major)
University Of Dundee

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  39nMAssay Description:Inhibition of Leishmania major recombinant PTR1More data for this Ligand-Target Pair
TargetPteridine reductase 1(Leishmania major)
University Of Dundee

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  180nMAssay Description:Inhibition of Leishmania major PTR1 by spectrophotometric assayMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  330nMAssay Description:Thermodynamic Dissociation Constant for compound-Tyr31-dihydrofolate reductase (DHFR) complex at pH 9.5More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetThymidylate synthase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  600nMAssay Description:Inhibition of human TS by spectrophotometric analysisMore data for this Ligand-Target Pair
TargetBifunctional purine biosynthesis protein ATIC(Homo sapiens (Human))
Wayne State University School Of Medicine

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataKi:  880nMAssay Description:Inhibition of human full length N-terminal His-tagged ATIC expressed in Chinese Hamster MTXRII-OuaR2-4 R2 cells assessed as reduction in THF formatio...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMedDrugBank

TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  2.40E+3nM ΔG°:  -32.1kJ/molepH: 5.5 T: 2°CAssay Description:Thermodynamic Dissociation Constant for compound-Val31-dihydrofolate reductase (DHFR) complex at pH 7More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetReduced folate transporter(Homo sapiens (Human))
The Institute Of Cancer Research

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  3.50E+3nMAssay Description:Affinity towards RFC measured by the inhibition of [3H]-MTX uptakeMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  3.90E+3nMAssay Description:Binding affinity of the compound towards Dihydrofolate reductase derived from L1210 cells using [3H]- MTX as the radioligandMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  3.90E+3nMAssay Description:Binding affinity of the compound towards Dihydrofolate reductase derived from L1210 cells using [3H]- MTX as the radioligandMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  4.42E+3nMAssay Description:Inhibition of human dihydrofolate reductaseMore data for this Ligand-Target Pair
TargetThymidylate synthase(Mus musculus)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  4.50E+4nMAssay Description:Inhibitory activity against thymidylate synthase in murine tumor cellsMore data for this Ligand-Target Pair
TargetATP-binding cassette sub-family C member 3(Rattus norvegicus)
The University Of Tokyo

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  9.00E+4nMAssay Description:TP_TRANSPORTER: inhibition of E217betaG uptake in membrane vesicles from Mrp3-expressing Sf9 cellsMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetGlucose-6-phosphate 1-dehydrogenase(Homo sapiens (Human))
Ataturk University

LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  9.10E+4nM IC50:  1.14E+5nMAssay Description:Ketotifen, dacarbazine, thiocolchicoside, meloxicam, methotrexate, furosemide, olanzapine, methylprednizolone acetate, paricalcitol, ritodrine hydroc...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
Target6-phosphogluconate dehydrogenase, decarboxylating(Homo sapiens (Human))
Ataturk University

LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  1.37E+5nM IC50:  1.42E+5nMAssay Description:In order to determine the effects of some drugs on human 6PGD, concentrations of ketotifen (0.0018-0.0282 mM), dacarbazine (0.0049-0.054 mM), meloxic...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMedDrugBank

TargetFolylpolyglutamate synthase, mitochondrial(Mus musculus)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  1.66E+5nMAssay Description:Compound was tested for its ability to inhibit Folyl-polyglutamate synthase from mouse liverMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  1.70E+5nMAssay Description:Thermodynamic Dissociation Constant for compound-Phe31-dihydrofolate reductase (DHFR) complex at pH 8.5More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetSolute carrier organic anion transporter family member(Danio rerio (Zebrafish))
Rudjer Boskovic Institute

LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  2.22E+5nMAssay Description:In the inhibition experiments, the cells were preincubated for 20 s with test compounds, followed by a 5-min incubation with [3H]E3S (5 nM) or 30-min...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetATP-binding cassette sub-family C member 2(Rattus norvegicus)
Toho University School Of Pharmaceutical Sciences

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  2.36E+5nMAssay Description:TP_TRANSPORTER: inhibition of DNP-SG uptake in bile canalicular membrane vesicles from SD ratMore data for this Ligand-Target Pair
In DepthDetails PubMed
TargetATP-binding cassette sub-family C member 2(Rattus norvegicus)
Toho University School Of Pharmaceutical Sciences

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  3.26E+5nMAssay Description:TP_TRANSPORTER: inhibition of L-MTX uptake in bile canalicular membrane vesicles from SD ratMore data for this Ligand-Target Pair
In DepthDetails PubMed
TargetATP-binding cassette sub-family C member 2(Rattus norvegicus)
Toho University School Of Pharmaceutical Sciences

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataKi:  4.62E+5nMAssay Description:TP_TRANSPORTER: inhibition of DNP-SG uptake in bile canalicular membrane vesicles from SD ratMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetSolute carrier family 22 member 20(Mus musculus)
University Of California

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  5.97E+5nMAssay Description:Inhibition of mouse Oat6-mediated [3H]ES uptake in Xenopus oocytes after 1 hrMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetSolute carrier family 22 member 6(Mus musculus)
University Of California

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  8.91E+5nMAssay Description:Inhibition of mouse Oat1-mediated [3H]PAH uptake in Xenopus oocytes after 1 hrMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetABC-type glutathione-S-conjugate transporter(Homo sapiens (Human))
The University Of Tokyo

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKi:  1.20E+6nMAssay Description:TP_TRANSPORTER: inhibition of E217betaG uptake in membrane vesicle from MRP3-expressing Sf9 cellsMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  0nMAssay Description:Concentration required to inhibit Lactobacillus casei derived Dihydrofolate reductase activity by 50%More data for this Ligand-Target Pair
TargetMethionine synthase(Homo sapiens (Human))
Peking University

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  0nMAssay Description:Inhibition of human Cobalamin-dependent methionine synthase isolated from HL-60 cells using methyltetrahydrofolate as substrate incubated for 10 mins...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  0.700nMAssay Description:Inhibitory concentration of the compound against CCRF-CEM leukemic cell DHFR(Dihydro folate reductase).More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  0.820nMAssay Description:Inhibitory concentration against dihydrofolate reductase (DHFR) enzyme isolated from CCRF-CEM human leukemia cells.More data for this Ligand-Target Pair
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Plasmodium falciparum (isolate K1 / Thailand))
Vyera Pharmaceuticals

US Patent
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  0.891nMAssay Description:TBDMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1nMAssay Description:Inhibition of human recombinant DHFRMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Pneumocystis carinii)
Indiana University School Of Medicine

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1nMAssay Description:Inhibition of Pneumocystis carinii (pc) Dihydrofolate reductaseMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Pneumocystis carinii)
Indiana University School Of Medicine

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1nMAssay Description:Inhibition of P. carinii dihydrofolate reductaseMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Pneumocystis carinii)
Indiana University School Of Medicine

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1nMAssay Description:Inhibitory concentration of the compound for DHFR in Pneumocystis cariniiMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Pneumocystis carinii)
Indiana University School Of Medicine

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1nMAssay Description:Inhibition against Dihydrofolate reductase in Pneumocystis cariniiMore data for this Ligand-Target Pair
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Trypanosoma cruzi)
Vyera Pharmaceuticals

US Patent
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  1.10nMAssay Description:TBDMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1.18nMAssay Description:Inhibitory activity against Dihydrofolate reductase (DHFR) isolated from CCRF-CEM human leukemia cells in experiment 1More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1.20nMAssay Description:Concentration required for 50% inhibition against dihydrofolate reductase of S. faeciumMore data for this Ligand-Target Pair
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Trypanosoma brucei brucei)
Vyera Pharmaceuticals

US Patent
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  1.29nMAssay Description:TBDMore data for this Ligand-Target Pair
In DepthDetails US Patent
TargetDihydrofolate reductase(Pneumocystis carinii)
Indiana University School Of Medicine

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1.30nMAssay Description:Inhibitory activity of the compound was determined against dihydrofolate reductase in Pneumocystis carinii at 37 cetigrade.More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1.30nMAssay Description:Compound was tested for the inhibition of dihydrofolate reductase in Bovine liverMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Pneumocystis carinii)
Indiana University School Of Medicine

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1.30nMAssay Description:Inhibitory activity against Pneumocystis carinii dihydrofolate reductase (in 90 microM dihydrofolic acid)More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Pneumocystis carinii)
Indiana University School Of Medicine

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1.30nMAssay Description:Inhibitory activity against Dihydrofolate reductase from Pneumocystis carinii was evaluated using 90 microM dihydrofolic acid as substrateMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1.40nMAssay Description:Inhibitory activity against Dihydrofolate reductase (DHFR) isolated from CCRF-CEM human leukemia cells in experiment 2More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1.40nMAssay Description:Inhibitory activity against Dihydrofolate reductase (DHFR) isolated from CCRF-CEM human leukemia cells in experiment 2More data for this Ligand-Target Pair
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Toxoplasma gondii)
University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1.40nMAssay Description:Inhibitory activity against Dihydrofolate reductase from Toxoplasma gondii was evaluated using 90 microM dihydrofolic acid as substrateMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1.5nMAssay Description:Inhibition of dihydrofolate reductase (unknown origin) using dihydrofolic acid as substrate by spectrophotometryMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1.60nMAssay Description:Inhibition of human DHFR using 45 uM DHF as substrate by spectrophotometryMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1.70nMAssay Description:In vitro inhibitory concentration against beef liver dihydrofolate reductaseMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1.70nMAssay Description:Inhibition of human recombinant DHFRMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  2nMAssay Description:Ability to inhibit recombinant human dihydrofolate reductase.More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  2nMAssay Description:Inhibition of human DHFR assessed as reduction in consumption of NADPH using DHF as substrate preincubated for 1 min followed by DHF addition and mea...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  2.10nMAssay Description:Inhibition of human dihydrofolate reductase by spectrophotometric analysisMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  2.20nMAssay Description:Inhibitory activity of the compound against recombinant sf dihydrofolate reductase(in 50 microM dihydrofolic acid)More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  2.20nMAssay Description:Inhibitory activity of the compound against recombinant ec dihydrofolate reductase(in 50 microM dihydrofolic acid)More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  2.29nMAssay Description:TBDMore data for this Ligand-Target Pair
In DepthDetails US Patent
TargetDihydrofolate reductase(Rattus norvegicus (rat))
Duquesne University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  2.5nMAssay Description:Inhibitory activity against rat liver dihydrofolate reductase (in 90 microM dihydrofolic acid)More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Rattus norvegicus (rat))
Duquesne University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  2.5nMAssay Description:Inhibitory activity against Dihydrofolate reductase from rat liver was evaluated using 90 microM dihydrofolic acid as substrateMore data for this Ligand-Target Pair
TargetThymidylate synthase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  2.64nMAssay Description:Compound was evaluated for inhibitory effect on the thymidylate synthase (TS) of Lactobacillus caseiMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  2.70nMAssay Description:Inhibitory activity against dihydrofolate reductase of Streptococcus faeciumMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  2.70nMAssay Description:In vitro inhibition of human dihydrofolate reductase (DHFR)More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Rattus norvegicus (rat))
Duquesne University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  3nMAssay Description:Inhibition against Dihydrofolate reductase in rat liverMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Rattus norvegicus (rat))
Duquesne University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  3nMAssay Description:Inhibitory concentration of the compound for DHFR in rat liverMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Rattus norvegicus (rat))
Duquesne University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  3nMAssay Description:Inhibition of rat liver Dihydrofolate reductaseMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  3nMAssay Description:Inhibition of MTX-resistant Lactobacillus casei dihydrofolate reductaseMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Rattus norvegicus (rat))
Duquesne University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  3nMAssay Description:Inhibition of Dihydrofolate reductase of rat liverMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  3nMAssay Description:Inhibition of Lactobacillus casei ATCC 7469 dihydrofolate reductaseMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Escherichia coli)
University of Prince Edward Island

LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  3nMpH: 7.0 T: 2°CAssay Description:The DHFR inhibition observed with each derivative was characterized by determination and comparison of the IC50 values. IC50 determinations were perf...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  3nMAssay Description:Inhibition of human DHFRMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  3.30nMAssay Description:Compound was evaluated in vitro for its ability to inhibit the Dihydrofolate reductase extracted from beef liver.More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  3.40nMAssay Description:Inhibition of bovine liver DHFR using dihydrofolic acid substrate by UV-visible spectrophotometryMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  3.40nMAssay Description:Inhibition activity using bovine dihydrofolate reductase (DHFR).More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  3.52nMAssay Description:Compound was evaluated for inhibitory effect on the dihydrofolate reductase (DHFR) of Lactobacillus casei.More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  3.90nMAssay Description:Compound was tested for its inhibitory activity on Recombinant Human Dihydrofolate Reductase.More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  4nMAssay Description:Inhibitory concentration of the compound for DHFR in recombinant humanMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  4.27nMAssay Description:TBDMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  4.60nMAssay Description:Inhibition of recombinant human DHFR expressed in Escherichia coli preincubated for 15 mins followed by addition of DHF as substrate and NADPH measur...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Rattus norvegicus (rat))
Duquesne University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  4.60nMAssay Description:Inhibition of rat liver dihydrofolate reductase assayed spectrophotometrically at 340 nMMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  4.70nMAssay Description:Inhibition of human DHFR expressed in Escherichia coli BL21 competent cells using DHF as substrate preincubated for 15 mins followed by substrate and...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  5nMAssay Description:Inhibition of recombinant human DHFR measured by spectrophotometric methodMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  5nMAssay Description:Inhibition of Lactobacillus casei DHFR by spectrophotometric analysisMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mycobacterium avium)
Oklahoma State University At Stillwater

LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  5.10nMpH: 7.0 T: 2°CAssay Description:Activity was measured as a change in absorbance over time at a wavelength of 340 nm (A340), so as to monitor the disappearance of NADPH. After incuba...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  5.37nMAssay Description:TBDMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  5.37nMAssay Description:TBDMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  5.37nMAssay Description:TBDMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  5.37nMAssay Description:TBDMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  6nMAssay Description:Inhibitory concentration of the compound for DHFR in Lactobacillus caseiMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  6nMAssay Description:Inhibitory concentration against isolated Escherichia coli Dihydrofolate reductaseMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  6nMAssay Description:Inhibition of Lactobacillus casei DHFRMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  6nMAssay Description:Inhibition of DHFR in human KB cells expressing RFC/FRalpha/PCFT assessed as reduction in cell growth after 96 hrs by Cell-Titer Blue assayMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  6nMAssay Description:Inhibitory activity against Escherichia coli (ec) Dihydrofolate reductaseMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  6nMAssay Description:inhibitory concentration of the compound against Lactobacillus casei DHFR(Dihydro folate reductase).More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  6.60nMAssay Description:Compound was tested for the inhibition of dihydrofolate reductase in P388 cellsMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Escherichia coli)
University of Prince Edward Island

LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  6.60nMpH: 7.4 T: 2°CAssay Description:DHFRs were assayed spectrophotometrically in the reaction buffer solution containing dihydrofolate. The reaction was initiated with an amount of enzy...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Escherichia coli)
University of Prince Edward Island

LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  6.60nMpH: 7.4 T: 2°CAssay Description:Enzymatic activities were determined by monitoring the oxidation of NADPH at 340 nm using a 96-well microtiter plate reading spectrophotometer.More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  6.70nMAssay Description:Inhibition of recombinant human DHFR using DHF as substrate measured every 5 sec over 6 mins in presence of NADPH by UV-Visible spectrophotometric me...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  7nMAssay Description:Inhibition of Escherichia coli DHFRMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  7nMpH: 7.4Assay Description:Inhibition of Escherichia coli dihydrofolate reductase at 37 degree C pH 7.4More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  7nMAssay Description:Compound was evaluated as inhibitor of Escherichia coli Dihydrofolate reductaseMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  7.10nMAssay Description:Inhibition of recombinant human DHFR assessed as reduction in consumption of NADPH using DHF as substrate measured every 30 secs over 6 mins by spect...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  7.90nMAssay Description:Inhibition of recombinant human DHFR assessed as reduction in conversion of DHF to THF measured every 30 secs for 6 mins by UV-Vis spectrophotometric...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  7.90nMAssay Description:Inhibition of human recombinant dihydrofolate reductase using dihydrofolate as substrate measured every 30 secs over 6 mins by UV/visible spectrophot...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  8nMAssay Description:Concentration required for 50% inhibition against dihydrofolate reductase of L. caseiMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  8nMAssay Description:Inhibition of bovine liver DHFR preincubated for 2 mins followed by addition of FH2 as substrate and NADPHMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  8nMAssay Description:Inhibition of bovine liver DHFRMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  8nMAssay Description:Inhibitory activity against dihydrofolate reductase derived from Lactobacillus caseiMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mycobacterium tuberculosis)
Institute Of Chemical Technology

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  8.30nMAssay Description:Inhibition of recombinant Mycobacterium tuberculosis DHFR using 45 uM DHF as substrate by spectrophotometryMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  8.5nMAssay Description:Inhibitory activity against dihydrofolate reductase of Lactobacillus caseiMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Escherichia coli)
University of Prince Edward Island

LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  8.80nMAssay Description:DHFRs were assayed spectrophotometrically in the reaction buffer solution containing dihydrofolate. The reaction was initiated with an amount of enzy...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Escherichia coli)
University of Prince Edward Island

LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  8.80nMAssay Description:DHFRs were assayed spectrophotometrically in the reaction buffer solution containing dihydrofolate. The reaction was initiated with an amount of enzy...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mycobacterium tuberculosis)
Institute Of Chemical Technology

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  8.80nMAssay Description:Inhibition of Mycobacterium tuberculosis H37Rv DHFR expressed in Escherichia coli BL21(DE3) assessed as reduction in consumption of NADPH using DHF a...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  8.80nMAssay Description:Inhibition of Escherichia coli DHFRMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  9nMAssay Description:Inhibition of human DHFRMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  9nMAssay Description:Inhibition of DHFR using dihydrofolic acid substrateMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  9nMAssay Description:Inhibition of Escherichia coli dihydrofolate reductase (DHFR)More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  9nMAssay Description:Inhibitory concentration against Escherichia coli Dihydrofolate reductaseMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  9.60nMAssay Description:Ability to inhibit Lactobacillus casei dihydrofolate reductase in vitro was determinedMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  9.60nMAssay Description:Ability to inhibit Lactobacillus casei dihydrofolate reductase in vitro was determinedMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  10nMAssay Description:Compound was evaluated for the inhibition of dihydrofolate reductase (DHFR)derived from Lactobacillus caseiMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  10nMAssay Description:Compound was evaluated for the inhibition of dihydrofolate reductase (DHFR) derived from Lactobacillus casei ATCC 7469More data for this Ligand-Target Pair
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  10nMAssay Description:Inhibition of TS/AICARFTase/GARFTase in human KB cells assessed as reduction in cell proliferation in folate free medium after 72 hrs in presence of ...More data for this Ligand-Target Pair
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Toxoplasma gondii)
University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  11nMAssay Description:Inhibition of Toxoplasma gondii dihydrofolate reductaseMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  11nMAssay Description:Inhibitory concentration against Lactobacillus casei Dihydrofolate reductaseMore data for this Ligand-Target Pair
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Toxoplasma gondii)
University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  11nMpH: 7.4 T: 2°CAssay Description:DHFRs were assayed spectrophotometrically in the reaction buffer solution containing dihydrofolate. The reaction was initiated with an amount of enzy...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  11nMAssay Description:Tested for inhibitory concentration against human dihydrofolate reductase(DHFR)More data for this Ligand-Target Pair
TargetReduced folate transporter(Homo sapiens (Human))
The Institute Of Cancer Research

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  12nMAssay Description:Inhibition of RFC (unknown origin) expressed in Chinese hamster PC43-10 cells assessed as cell growth inhibition incubated up to 96 hrs by Celltiter-...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  12nMAssay Description:In vitro inhibition of Dihydrofolate reductase activity against Lactobacillus casei enzymeMore data for this Ligand-Target Pair
TargetReduced folate transporter(Homo sapiens (Human))
The Institute Of Cancer Research

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  12nMAssay Description:Binding affinity to human RFC expressed in Chinese hamster PC43-10 cells assessed as antiproliferative activity measured as reduction in cell viabili...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  13.8nMAssay Description:Inhibitory concentration against isolated Pneumocystis carinii DHFR (Dihydrofolate reductase)More data for this Ligand-Target Pair
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Toxoplasma gondii)
University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  14nMAssay Description:Inhibition of T. gondii Dihydrofolate reductaseMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Toxoplasma gondii)
University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  14nMAssay Description:Inhibition against Dihydrofolate reductase in Toxoplasma gondiiMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Toxoplasma gondii)
University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  14nMAssay Description:Inhibitory activity against Toxoplasma gondii dihydrofolate reductase (in 90 microM dihydrofolic acid)More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Toxoplasma gondii)
University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  14nMAssay Description:Inhibition of Toxoplasma gondii (tc) Dihydrofolate reductaseMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Toxoplasma gondii)
University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  14nMAssay Description:Inhibitory concentration of the compound for DHFR in Toxoplasma gondiiMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  14.5nMAssay Description:Inhibitory concentration against isolated human Dihydrofolate reductaseMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  15nMAssay Description:Inhibitory activity of the compound against dihydrofolate reductase in Lactobacillus casei was determined (glutamate resedue 3)More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  15nMAssay Description:Inhibitory activity against dihydrofolate reductase in Lactobacillus casei was determined (glutamate residue 4)More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bacillus anthracis)
Oklahoma State University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  15nMAssay Description:Inhibition of Bacillus anthracis DHFRMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bacillus anthracis)
Oklahoma State University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  15nMAssay Description:Inhibition of Bacillus anthracis DHFRMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bacillus anthracis)
Oklahoma State University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  15nMpH: 7.1 T: 2°CAssay Description:Activity was measured as a change in absorbance over time at a wavelength of 340 nm (A340), so as to monitor the disappearance of NADPH. After incuba...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bacillus anthracis)
Oklahoma State University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  15.1nMpH: 7.0 T: 2°CAssay Description:Activity was measured as a change in absorbance over time at a wavelength of 340 nm (A340), so as to monitor the disappearance of NADPH. After incuba...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  16nMAssay Description:Evaluated for inhibition of dihydrofolate reductase (DHFR) from Lactobacillus caseiMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  16nMAssay Description:Inhibitory activity against dihydrofolate reductase of Lactobacillus caseiMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  16nMAssay Description:Inhibitory activity of the compound against dihydrofolate reductase in Lactobacillus casei was determined (glutamate resedue 6)More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  16nMAssay Description:Inhibitory activity of the compound against dihydrofolate reductase in Lactobacillus casei was determined (glutamate resedue 5)More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  16nMAssay Description:Inhibitory activity against dihydrofolate reductase in Lactobacillus casei was determined (glutamate residue 4)More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  16nMAssay Description:Inhibitory activity of the compound against dihydrofolate reductase in Lactobacillus casei was determined (glutamate resedue 2)More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  18nMpH: 7.5Assay Description:The recombinant human (rh) DHFR was purchased from Sigma-Aldrich (Shanghai, China). All rhDHFR enzymes were assayed spectrophotometrically in a solut...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Gallus gallus (Chicken))
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  19nMAssay Description:Compound was evaluated for the inhibition of dihydrofolate reductase (DHFR) derived from Chicken liverMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  20nMAssay Description:DHFRs were assayed spectrophotometrically in the reaction buffer solution containing dihydrofolate. The reaction was initiated with an amount of enzy...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  20nMAssay Description:DHFRs were assayed spectrophotometrically in the reaction buffer solution containing dihydrofolate. The reaction was initiated with an amount of enzy...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  20nMAssay Description:Compound was tested for its inhibitory concentration to inhibit the enzyme Dihydro Folate Reductase (DHFR) from murine L1210 leukemia cells.More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  20nMAssay Description:Inhibition of human recombinant DHFRMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  20nMAssay Description:Inhibition of human recombinant DHFRMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  20nMAssay Description:Inhibition of human recombinant DHFR expressed in Escherichia coli by spectrophotometric analysisMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  20nMAssay Description:Compound was tested for its inhibitory activity against dihydrofolate reductase (DHFR), isolated from MTX-resistant WI-L2 cellsMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  20nMAssay Description:Inhibition of human DHFRMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  20nMAssay Description:Inhibition of DHFR in human KB cells expressing RFC/FRalpha/PCFT assessed as reduction in cell growth after 96 hrs in presence of folic acid by Cell-...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  22nMAssay Description:Inhibition of human recombinant DHFRMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  22nM EC50:  13.3nMAssay Description:Enzymatic activities were determined by monitoring the oxidation of NADPH at 340 nm using a 96-well microtiter plate reading spectrophotometer.More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  22nM EC50:  12.5nMpH: 7.4 T: 2°CAssay Description:DHFRs were assayed spectrophotometrically in the reaction buffer solution containing dihydrofolate. The reaction was initiated with an amount of enzy...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  22nMAssay Description:Inhibition of rat hippocampal Gamma-aminobutyric acid type B receptor (weak agonist)More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  22nMAssay Description:Compound was evaluated as inhibitor of Lactobacillus casei Dihydrofolate reductaseMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  22nMAssay Description:Compound was evaluated as inhibitor of human Dihydrofolate reductaseMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  22nMAssay Description:Inhibition of rh dihydrofolate reductase (DHFR)More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  22nMAssay Description:Inhibitory activity against recombinant human Dihydrofolate reductaseMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  22nMAssay Description:Inhibition of human DHFRMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  22nMpH: 7.4Assay Description:Inhibition of human dihydrofolate reductase at 37 degree C pH 7.4More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  22nMAssay Description:Inhibition of Lactobacillus casei DHFRMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  22nMpH: 7.4Assay Description:Inhibition of human DHFR at 30 degC under pH 7.4 by spectrophotometryMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  22nMAssay Description:Inhibitory concentration against isolated Toxoplasma gondii DHFR (dihydrofolate reductase)More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  22nMAssay Description:Inhibitory concentration against recombinant human (rh) Dihydrofolate reductaseMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  22nMAssay Description:Inhibition of human dihydrofolate reductaseMore data for this Ligand-Target Pair
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Toxoplasma gondii)
University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  22nMAssay Description:Inhibitory activity of the compound against Toxoplasma gondii dihydrofolate reductase (in 50 microM dihydrofolic acid)More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  22nMpH: 7.4Assay Description:Inhibition of Lactobacillus casei dihydrofolate reductase at 37 degree C pH 7.4More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  22nMAssay Description:Inhibition of human DHFR by spectrophotometryMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  24nMAssay Description:Inhibition of dihydrofolate reductase (DHFR) from human cells (WI-L2/M4).More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bacillus cereus (ATCC 14579))
University of Connecticut At Storrs

LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  24nMpH: 7.0 T: 2°CAssay Description:Enzyme activity assays were performed by monitoring the rate of enzyme-dependent NADPH consumption at an absorbance of 340 nm over a period of severa...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  24nMAssay Description:Compound was evaluated for Dihydrofolate Reductase (DHFR) inhibitionMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  24.3nMpH: 7.0 T: 2°CAssay Description:Activity was measured as a change in absorbance over time at a wavelength of 340 nm (A340), so as to monitor the disappearance of NADPH. After incuba...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  25nMAssay Description:Inhibition of dihydrofolate reductase(DHFR) from L1210/R81 cellsMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  25nMAssay Description:Inhibition of dihydrofolate reductase (DHFR) from mouse cells (L1210/R71).More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  25nMAssay Description:Inhibition of recombinant human DHFR using dihydrofolic acid as substrate in presence of NADPH after 15 mins by microplate reader analysisMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  25nMAssay Description:Inhibition of dihydrofolate reductase (DHFR) from murine leukemia cellsMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  25nMAssay Description:Tested for inhibition against purified Dihydrofolate reductase from L1210 murine leukemia cellsMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  25nMAssay Description:Ability to inhibit purified Dihydrofolate reductase from human leukemic lymphoblasts was determined spectrophotometrically at 340 nM.More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  30nMAssay Description:Inhibitory activity of the compound towards dihydrofolate reductase derived from human manca leukemia cellsMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  31nMAssay Description:Inhibition of recombinant human DHFR using dihydrofolate as substrate after 15 mins in presence of NADPHMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  32.9nMAssay Description:Inhibition of human recombinant DHFR by spectrophotometryMore data for this Ligand-Target Pair
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Toxoplasma gondii)
University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  33nMAssay Description:DHFRs were assayed spectrophotometrically in the reaction buffer solution containing dihydrofolate. The reaction was initiated with an amount of enzy...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Toxoplasma gondii)
University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  33nMAssay Description:Inhibition of Toxoplasma gondii DHFRMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Toxoplasma gondii)
University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  33nMAssay Description:DHFRs were assayed spectrophotometrically in the reaction buffer solution containing dihydrofolate. The reaction was initiated with an amount of enzy...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Toxoplasma gondii)
University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  33nMAssay Description:Inhibition of Toxoplasma gondii DHFRMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  35nMAssay Description:Inhibition of DHFR using folic acid substrateMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  35nMAssay Description:Inhibition of recombinant human DHFR using dihydrofolate as substrate after 15 mins in presence of NADPHMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mycobacterium tuberculosis)
Institute Of Chemical Technology

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  35nMAssay Description:Inhibition of Mycobacterium tuberculosis His-tagged DHFR assessed as reduction in consumption of NADPH using DHF as substrate measured every 30 secs ...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  35nMAssay Description:Compound was tested for its ability to inhibit purified dihydrofolate reductase(DHFR) from L1210/R81 cellsMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  35nMAssay Description:Compound was tested for its ability to inhibit dihydrofolate reductase purified from murine L1210 cellsMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  38nMAssay Description:Inhibitory activity against recombinant human dihydrofolate reductase(in 50 microM dihydrofolic acid)More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  40nMAssay Description:Inhibitory concentration of the compound against recombinant human DHFR(Dihydro folate reductase).More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  44nMAssay Description:Inhibitory concentration against isolated Lactobacillus casei Dihydrofolate reductaseMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  50nMAssay Description:Inhibitory activity of the compound towards dihydrofolate reductase in L1210 murine leukemia cell linesMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  55nMAssay Description:Inhibitory activity of the compound against recombinant lc dihydrofolate reductase(in 50 microM dihydrofolic acid)More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  61nMAssay Description:Inhibition of recombinant human DHFR expressed in Escherichia coli incubated for 15 mins and measured by ELISAMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  66nMAssay Description:Inhibitory activity against human dihydrofolate reductaseMore data for this Ligand-Target Pair
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Toxoplasma gondii)
University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  71nMAssay Description:Inhibition of Toxoplasma gondii TS-DHFR expressed in Escherichia coli BL21 preincubated for 15 mins followed by addition of DHF as substrate and NADP...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Mus musculus (Mouse))
Cornell University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  73nMAssay Description:compound was evaluated for the inhibitory activity against Dihydrofolate reductase in permeabilised L1210 cells.More data for this Ligand-Target Pair
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Toxoplasma gondii)
University

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  77.6nMAssay Description:TBDMore data for this Ligand-Target Pair
In DepthDetails US Patent
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Toxoplasma gondii)
University

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  78nMAssay Description:Inhibition of Toxoplasma gondii DHFR-TS expressed in Escherichia coli BL21 competent cells using DHF as substrate preincubated for 15 mins followed b...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  80nMAssay Description:Inhibition of bovine DHFR after 2 mins by spectrophotometryMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  80nMAssay Description:Inhibition of bovine liver DHFR measured for every 1 min of 10 minsMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  80nMpH: 7.4 T: 2°CAssay Description:The assay mixture contained 50 μM Tris-HCl buffer (pH 7.4), 50 μM NADPH, 10 μl DMSO or the same volume of DMSO solution containing the...More data for this Ligand-Target Pair
In DepthDetails PDB3D3D Structure (crystal)
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  80nMAssay Description:Inhibition of human DHFR after 2 minsMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  80nMAssay Description:Inhibition of bovine liver DHFR measured every 1 min for 10 minsMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  80nMAssay Description:Inhibition of bovine DHFR after 2 minsMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  83nMAssay Description:Inhibition of recombinant human DHFR using dihydrofolate as substrate in presence of NADPHMore data for this Ligand-Target Pair
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Plasmodium falciparum (isolate K1 / Thailand))
Vyera Pharmaceuticals

US Patent
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  84nMAssay Description:Inhibition of Plasmodium falciparum DHFRMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  88nMAssay Description:Inhibition of DHFR (unknown origin) incubated for 2 to 5 mins by absorbance based analysisMore data for this Ligand-Target Pair
TargetFolate receptor beta(Homo sapiens (Human))
Duquesne University

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  106nMAssay Description:Binding affinity to human FR-beta receptor expressed in Chinese hamster D4 cells assessed as antiproliferative activity measured as reduction in cell...More data for this Ligand-Target Pair
TargetFolate receptor beta(Homo sapiens (Human))
Duquesne University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  106nMAssay Description:Inhibition of FRbeta (unknown origin) expressed in Chinese hamster D4 cells assessed as cell growth inhibition incubated up to 96 hrs by Celltiter-bl...More data for this Ligand-Target Pair
TargetFolate receptor alpha(Homo sapiens (Human))
Duquesne University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  114nMAssay Description:Inhibition of FRalpha (unknown origin) expressed in Chinese hamster RT16 cells assessed as cell growth inhibition incubated up to 96 hrs by Celltiter...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMedDrugBank

TargetFolate receptor alpha(Homo sapiens (Human))
Duquesne University

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  114nMAssay Description:Binding affinity to human FR-alpha receptor expressed in Chinese hamster RT16 cells assessed as antiproliferative activity measured as reduction in c...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMedDrugBank

TargetProton-coupled folate transporter(Homo sapiens (Human))
Duquesne University

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  121nMAssay Description:Binding affinity to human PCFT expressed in Chinese hamster R2/PCFT4 cells assessed as antiproliferative activity measured as reduction in cell viabi...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMedDrugBank

TargetProton-coupled folate transporter(Homo sapiens (Human))
Duquesne University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  121nMAssay Description:Inhibition of PCFT (unknown origin) expressed in Chinese hamster R2/PCFT4 cells assessed as cell growth inhibition incubated up to 96 hrs by Celltite...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMedDrugBank

TargetFolate receptor beta(Homo sapiens (Human))
Duquesne University

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  211nMAssay Description:Binding affinity to human FR-beta receptor expressed in Chinese hamster D4 cells assessed as antiproliferative activity measured as reduction in cell...More data for this Ligand-Target Pair
TargetFolate receptor beta(Homo sapiens (Human))
Duquesne University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  211nMAssay Description:Inhibition of FRbeta (unknown origin) expressed in Chinese hamster D4 cells assessed as cell growth inhibition incubated up to 96 hrs in presence of ...More data for this Ligand-Target Pair
TargetFolate receptor alpha(Homo sapiens (Human))
Duquesne University

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  216nMAssay Description:Binding affinity to human FR-alpha receptor expressed in Chinese hamster RT16 cells assessed as antiproliferative activity measured as reduction in c...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMedDrugBank

TargetFolate receptor alpha(Homo sapiens (Human))
Duquesne University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  461nMAssay Description:Inhibition of FRalpha (unknown origin) expressed in Chinese hamster RT16 cells assessed as cell growth inhibition incubated up to 96 hrs in presence ...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMedDrugBank

TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  720nMAssay Description:Inhibitory activity against purified human dihydrofolate reductase (DHFR) in human leukemia cells (CCRF-CEM)More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Pneumocystis carinii)
Indiana University School Of Medicine

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  880nMAssay Description:Inhibitory activity was determined against dihydrofolate reductase in Pneumocystis carinii .More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  1.00E+3nMAssay Description:Inhibition of human dihydrofolate reductase using dihydrofolic acid as substrate preincubated for 1 hr in presence of NADPH followed by substrate add...More data for this Ligand-Target Pair
TargetToll-like receptor 4(Homo sapiens (Human))
Hainan Normal University

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  1.04E+3nMAssay Description:Inhibition of TLR4 (unknown origin) by sandwich ELISA methodMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1.50E+3nMAssay Description:Inhibition of human DHFR assessed as conversion of dihydrofolic acid to tetrahydrfolic acid by enzyme immunoassay in presence of NADPHMore data for this Ligand-Target Pair
TargetThymidylate synthase(Escherichia coli)
Duquesne University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1.80E+3nMAssay Description:Inhibitory activity against Escherichia coli thymidylate synthaseMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetFolylpolyglutamate synthase, mitochondrial(Homo sapiens (Human))
State University Of New York

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  3.20E+3nMAssay Description:Compound was evaluated for the inhibitory activity against Folyl-polyglutamate synthase from CCRF-CEM human leukemia cells.More data for this Ligand-Target Pair
In DepthDetails ArticlePubMedDrugBank

TargetThymidylate synthase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  3.60E+3nMAssay Description:Inhibitory activity against human thymidylate synthaseMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  4.40E+3nMAssay Description:Inhibitory activity against recombinant Escherichia coli dihydrofolate reductaseMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Toxoplasma gondii)
University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1.80E+4nMAssay Description:TS was assayed spectrophotometrically in the reaction buffer solution containing (6R, 6S)-5, 10-CH2H4folate. The reaction was initiated by the additi...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetThymidylate synthase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50: >1.80E+4nMAssay Description:Inhibition of human TS assessed as oxidation of tetrahydrofolate to dihydrofolate after 2 to 12 mins by spectrophotometryMore data for this Ligand-Target Pair
TargetThymidylate synthase(Escherichia coli)
Duquesne University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50: >1.80E+4nMAssay Description:Inhibition of Escherichia coli TS assessed as oxidation of tetrahydrofolate to dihydrofolate after 2 to 12 mins by spectrophotometryMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetBifunctional dihydrofolate reductase-thymidylate synthase(Toxoplasma gondii)
University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1.80E+4nMAssay Description:Inhibition of Toxoplasma gondii thymidylate synthaseMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetTrifunctional purine biosynthetic protein adenosine-3(Mus musculus)
Southern Research Institute

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50: >2.00E+4nMAssay Description:Inhibition of GAR transformylase from mammalian L1210 cellsMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetTrifunctional purine biosynthetic protein adenosine-3(Mus musculus)
Southern Research Institute

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50: >2.00E+4nMAssay Description:Inhibition of GAR transformylase from Lactobacillus caseiMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetTrifunctional purine biosynthetic protein adenosine-3(Homo sapiens (Human))
Sri International

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50: >2.00E+4nMAssay Description:Inhibitory activity against GAR transformylase of Lactobacillus caseiMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetBifunctional purine biosynthesis protein ATIC(Homo sapiens (Human))
Wayne State University School Of Medicine

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50: >2.00E+4nMAssay Description:Inhibitory activity against AICAR formyltransferase of Lactobacillus caseiMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMedDrugBank

TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  2.17E+4nMAssay Description:Inhibition of beef liver dihydrofolate reductase by free MTX in experiment 2More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  2.42E+4nMAssay Description:Concentration required for inhibition of beef liver dihydrofolate reductaseMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  2.64E+4nMAssay Description:Inhibition of beef liver dihydrofolate reductase by free MTX in experiment 1More data for this Ligand-Target Pair
TargetThymidylate synthase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  2.90E+4nMAssay Description:Inhibition of human thymidylate synthaseMore data for this Ligand-Target Pair
TargetThymidylate synthase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  2.90E+4nMpH: 7.4Assay Description:Inhibition of human thymidylate synthase at 30 degC under pH 7.4 by spectrophotometryMore data for this Ligand-Target Pair
TargetThymidylate synthase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  2.90E+4nMAssay Description:TS was assayed spectrophotometrically in the reaction buffer solution containing (6R, 6S)-5, 10-CH2H4folate. The reaction was initiated by the additi...More data for this Ligand-Target Pair
TargetTegument protein VP16(Human herpesvirus 1 (strain 17) (HHV-1) (Human her...)
The Scripps Research Institute Molecular Screening Center

Curated by PubChem BioAssay
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50: >3.59E+4nMAssay Description:Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Affiliation: The Scripps Research Institute, TSRI Assa...More data for this Ligand-Target Pair
In DepthDetails PCBioAssay
TargetNuclear receptor coactivator 1(Homo sapiens (Human))
The Scripps Research Institute Molecular Screening Center

Curated by PubChem BioAssay
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50: >3.59E+4nMAssay Description:Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Affiliation: The Scripps Research Institute, TSRI Assa...More data for this Ligand-Target Pair
In DepthDetails PCBioAssay
TargetNuclear receptor coactivator 3(Homo sapiens (Human))
The Scripps Research Institute Molecular Screening Center

Curated by PubChem BioAssay
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50: >3.59E+4nMAssay Description:Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Affiliation: The Scripps Research Institute, TSRI Assa...More data for this Ligand-Target Pair
In DepthDetails PCBioAssay
TargetThymidylate synthase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  4.00E+4nMAssay Description:Ability to inhibit Lactobacillus casei thymidylate synthase in vitro was determinedMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetThymidylate synthase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  7.50E+4nMAssay Description:In vitro inhibition of activity against Lactobacillus casei enzyme thymidylate synthaseMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  7.92E+4nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate (regiospecific coupling) of MTX (4 M/M of IgG)and rabbit IgG (NRG) in expe...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  7.92E+4nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate (regiospecific coupling) of MTX (4 M/M of IgG)and IgG1 in experiment 2More data for this Ligand-Target Pair
TargetThymidylate synthase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  8.60E+4nMAssay Description:Inhibitory activity against thymidylate synthase of Lactobacillus caseiMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  8.80E+4nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate (regiospecific coupling) of MTX (6 M/M of IgG)and rabbit IgG (NRG) in expe...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  8.80E+4nMAssay Description:Inhibition of beef liver dihydrofolate reductase by hydrazone-linked conjugate (regiospecific coupling) of MTX (6 M/M of IgG)and Dal K-20 in experime...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  8.80E+4nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate (regiospecific coupling) of MTX (7 M/M of IgG)and Dal K-20 in experiment 2More data for this Ligand-Target Pair
TargetThymidylate synthase(Escherichia coli)
Duquesne University

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  9.00E+4nMAssay Description:Inhibition of Escherichia coli thymidylate synthaseMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetThymidylate synthase(Escherichia coli)
Duquesne University

LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  9.00E+4nMAssay Description:TS was assayed spectrophotometrically in the reaction buffer solution containing (6R, 6S)-5, 10-CH2H4folate. The reaction was initiated by the additi...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  9.24E+4nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate (regiospecific coupling) of MTX (4 M/M of IgG)and rabbit IgG (NRG) in expe...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  9.24E+4nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate (regiospecific coupling) of MTX (4 M/M of IgG)and IgG1 in experiment 1More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  9.46E+4nMAssay Description:Inhibition of beef liver dihydrofolate reductase by hydrazone-linked conjugate of MTX (7 M/M of IgG)and Dal K-20 in experiment 2More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  9.90E+4nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate (regiospecific coupling) of MTX (7 M/M of IgG)and Dal K-20 in experiment 1More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  9.90E+4nMAssay Description:Inhibition of beef liver dihydrofolate reductase by hydrazone-linked conjugate (regiospecific coupling) of MTX (6 M/M of IgG)and Dal K-20 in experime...More data for this Ligand-Target Pair
TargetThymidylate synthase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1.00E+5nMAssay Description:Inhibition of Lactobacillus casei ATCC 7469 thymidylate synthaseMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetThymidylate synthase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50: >1.00E+5nMAssay Description:Inhibition of MTX-resistant Lactobacillus casei thymidylate synthetaseMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetThymidylate synthase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1.00E+5nMAssay Description:Compound was evaluated for the inhibition of thymidylate synthase (TS) derived from Lactobacillus caseiMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  1.01E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate (regiospecific coupling) of MTX (6 M/M of IgG)and rabbit IgG (NRG) in expe...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  1.07E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate of MTX (6 M/M of IgG)and IgG1 in experiment 2More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  1.10E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by hydrazone-linked conjugate (regiospecific coupling) of MTX (7 M/M of IgG) and rabbit IgG (NRG) in...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  1.10E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by hydrazone-linked conjugate (regiospecific coupling) of MTX (5M/M of IgG) and rabbit IgG (NRG) in ...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  1.14E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate of MTX (7 M/M of IgG)and Dal K-20 in experiment 2More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  1.16E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by hydrazone-linked conjugate of MTX (7 M/M of IgG)and Dal K-20 in experiment 1More data for this Ligand-Target Pair
TargetThymidylate synthase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1.20E+5nMAssay Description:Evaluated for inhibition of thymidylate synthase (TS) from Lactobacillus caseiMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetThymidylate synthase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1.20E+5nMAssay Description:Inhibitory activity against thymidylate synthase of Streptococcus faeciumMore data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  1.25E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate of MTX (6 M/M of IgG) and rabbit IgG (NRG) in experiment 2More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  1.27E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by hydrazone-linked conjugate of MTX (6 M/M of IgG) and rabbit IgG (NRG) in experiment 2More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  1.27E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate of MTX (4 M/M of IgG) and rabbit IgG (NRG) in experiment 2More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  1.29E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by hydrazone-linked conjugate (regiospecific coupling) of MTX (5M/M of IgG) and rabbit IgG (NRG) in ...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  1.32E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate of MTX (7 M/M of IgG)and Dal K-20 in experiment 1More data for this Ligand-Target Pair
TargetATP-binding cassette sub-family C member 2(Homo sapiens (Human))
Amgen

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50: >1.33E+5nMAssay Description:Inhibition of human MRP2 overexpressed in Sf9 cell membrane vesicles assessed as uptake of [3H]-estradiol-17beta-D-glucuronide in presence of ATP and...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetATP-binding cassette sub-family C member 3(Homo sapiens (Human))
Amgen

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50: >1.33E+5nMAssay Description:Inhibition of human MRP3 overexpressed in Sf9 insect cell membrane vesicles assessed as uptake of [3H]-estradiol-17beta-D-glucuronide in presence of ...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMedDrugBank

TargetATP-binding cassette sub-family C member 4(Homo sapiens (Human))
Amgen

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50: >1.33E+5nMAssay Description:Inhibition of human MRP4 overexpressed in Sf9 cell membrane vesicles assessed as uptake of [3H]-estradiol-17beta-D-glucuronide in presence of ATP and...More data for this Ligand-Target Pair
TargetBile salt export pump(Homo sapiens (Human))
Amgen

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50: >1.33E+5nMAssay Description:Inhibition of human BSEP overexpressed in Sf9 cell membrane vesicles assessed as uptake of [3H]-taurocholate in presence of ATP measured after 15 to ...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  1.34E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate of MTX (6 M/M of IgG)and IgG1 in experiment 1More data for this Ligand-Target Pair
TargetBile salt export pump(Homo sapiens (Human))
Amgen

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50: >1.35E+5nMAssay Description:Inhibition of human BSEP expressed in fall armyworm sf9 cell plasma membrane vesicles assessed as reduction in vesicle-associated [3H]-taurocholate t...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  1.36E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by hydrazone-linked conjugate (regiospecific coupling) of MTX (7 M/mL of IgG) and rabbit IgG (NRG) i...More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  1.38E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate of MTX (6 M/M of IgG) and rabbit IgG (NRG) in experiment 1More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  1.38E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by hydrazone-linked conjugate of MTX (7 M/M of IgG) and rabbit IgG (NRG) in experiment 2More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  1.45E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by hydrazone-linked conjugate of MTX (7 M/M of IgG) and rabbit IgG (NRG) in experiment 1More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  1.51E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate of MTX (4 M/M of IgG) and rabbit IgG (NRG) in experiment 1More data for this Ligand-Target Pair
TargetDihydrofolate reductase(Bos taurus (Cattle))
TBA

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50:  1.58E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by hydrazone-linked conjugate of MTX (6 M/M of IgG) and rabbit IgG (NRG) in experiment 1More data for this Ligand-Target Pair
TargetThymidylate synthase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1.70E+5nMAssay Description:Inhibitory concentration of the compound for TS in humanMore data for this Ligand-Target Pair
TargetThymidylate synthase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1.70E+5nMAssay Description:Inhibitory activity against thymidylate synthase of Lactobacillus caseiMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetThymidylate synthase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1.70E+5nMAssay Description:Inhibitory concentration of the compound against recombinant human TS (Thymidylate synthase).More data for this Ligand-Target Pair
TargetThymidylate synthase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  1.90E+5nMAssay Description:In vitro inhibitory concentration against Lactobacillus casei thymidylate synthetaseMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetThymidylate synthase(Lactobacillus casei)
TBA

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50:  6.00E+5nMAssay Description:Inhibitory activity against thymidylate synthase derived from Lactobacillus caseiMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetBile salt export pump(Homo sapiens (Human))
Amgen

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataIC50: >1.00E+6nMAssay Description:Inhibition of human BSEP expressed in baculovirus transfected fall armyworm Sf21 cell membranes vesicles assessed as reduction in ATP-dependent [3H]-...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetBile salt export pump(Homo sapiens (Human))
Amgen

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50: >1.00E+6nMAssay Description:Inhibition of human BSEP expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptakeMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetBile salt export pump(Rattus norvegicus)
Astrazeneca

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50: >1.00E+6nMAssay Description:Inhibition of Sprague-Dawley rat Bsep expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate up...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetProtein-arginine deiminase type-4(Homo sapiens (Human))
University Of South Carolina

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataIC50: >1.00E+7nMAssay Description:Inhibition of PAD4 by ABPP-based assayMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Escherichia coli)
University of Prince Edward Island

LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataEC50:  1nMAssay Description:CAII assays were performed at CEREP (paris). All other kinase assays were performed at Upstate (Dundee, UK).More data for this Ligand-Target Pair
TargetBcl-2-like protein 11(Homo sapiens (Human))
Broad Institute

Curated by PubChem BioAssay
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataEC50: >3.50E+5nMAssay Description:Keywords: apoptosis, BH3 domain, Bcl2-A1, BIM, caspase, cancer Primary Collaborator: Todd Golub, Broad Institute, golub@broadinstitute.org Assay Over...More data for this Ligand-Target Pair
In DepthDetails PCBioAssay
TargetVacuolar aminopeptidase 1(Saccharomyces cerevisiae)
Nmmlsc

Curated by PubChem BioAssay
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataEC50:  5.18E+3nMAssay Description:University of New Mexico Assay Overview: Assay Support: 1R03 MH086450-01 Project Title: Chemical Screen of TOR pathway GFP fusion proteins in S. ...More data for this Ligand-Target Pair
In DepthDetails PCBioAssay
TargetImportin subunit alpha-1(Homo sapiens (Human))
Broad Institute

Curated by PubChem BioAssay
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataEC50: >5.00E+5nMAssay Description:Keywords: Counterscreen, FRET assay, HTS, YIC probe, Dose response, Assay Overview: This is a fluorescence resonance energy transfer (FRET)-based b...More data for this Ligand-Target Pair
In DepthDetails PCBioAssay
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKd: <1nMAssay Description:Inhibitory activity against dihydrofolate reductaseMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKd:  3.20nMpH: 6.0Assay Description:Thermodynamic Dissociation Constant for compound-Val31-dihydrofolate reductase (DHFR) complex at pH 7More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKd:  2.30nMpH: 6.0Assay Description:Thermodynamic Dissociation Constant for compound-Val31-dihydrofolate reductase (DHFR) complex at pH 7More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKoff:  3.60E+7s-1Assay Description:Thermodynamic Dissociation Constant for compound-Val31-dihydrofolate reductase (DHFR) complex at pH 6More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKd:  1.10E+3nMpH: 9.5Assay Description:Thermodynamic Dissociation Constant for compound-Tyr31-dihydrofolate reductase (DHFR) complex at pH 6More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKon:  12M-1s-1Assay Description:Thermodynamic Dissociation Constant for compound-Val31-dihydrofolate reductase (DHFR) complex at pH 8.5More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKoff:  1.10E+7s-1Assay Description:Thermodynamic Dissociation Constant for compound-Val31-dihydrofolate reductase (DHFR) complex at pH 7More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKd:  140nMpH: 8.0Assay Description:Thermodynamic Dissociation Constant for compound-Val31-dihydrofolate reductase (DHFR) complex at pH 7More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKd:  0.0960nMpH: 8.0Assay Description:Association constant(Kon) at Tyr-31 of dihydrofolate reductase (DHFR)More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKd:  1.70E+3nMpH: 9.5Assay Description:Thermodynamic Dissociation Constant for compound-Val31-dihydrofolate reductase (DHFR) complex at pH 7More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKon:  26M-1s-1Assay Description:Binding constant(Ki) to dihydrofolate reductase (DHFR) Phe -31 was determinedMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKd:  31nMpH: 9.5Assay Description:Thermodynamic Dissociation Constant for compound-Val31-dihydrofolate reductase (DHFR) complex at pH 7More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKd:  0.0230nMpH: 6.0Assay Description:Thermodynamic Dissociation Constant for compound-Tyr31-dihydrofolate reductase (DHFR) complex at pH 6More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKoff:  1.50E+5s-1Assay Description:Thermodynamic Dissociation Constant for compound-Phe31-dihydrofolate reductase (DHFR) complex at pH 9.5More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKd:  84nMpH: 8.0Assay Description:Dissociation constant(Koff) at Phe-31 of dihydrofolate reductase (DHFR)More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Escherichia coli)
University Of Connecticut

Curated by ChEMBL
LigandPNGBDBM18050(2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)a...)
Affinity DataKon:  26M-1s-1Assay Description:Thermodynamic Dissociation Constant for compound-Val31-dihydrofolate reductase (DHFR) complex at pH 8More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetHigh mobility group protein B1(Homo sapiens (Human))
The Feinstein Institute For Medical Research

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataKd:  500nMAssay Description:Binding affinity to N-terminal 6His-tagged HMGB1 AI region (1 to 87 residues) (unknown origin) expressed in Escherichia coli JM109 by SPR assayMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetHigh mobility group protein B1(Homo sapiens (Human))
The Feinstein Institute For Medical Research

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataKd:  24nMAssay Description:Binding affinity to N-terminal 6His-tagged HMGB1 Bj region (88 to 181 residues) (unknown origin) expressed in Escherichia coli JM109 by SPR assayMore data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetDihydrofolate reductase(Homo sapiens (Human))
Universita` Degli Studi Di Sassari

Curated by ChEMBL
LigandPNGBDBM66082((2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methy...)
Affinity DataKd:  0.195nMAssay Description:Binding affinity against Dihydrofolate reductaseMore data for this Ligand-Target Pair
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