Affinity DataKi: 3.60nMAssay Description:Inhibition of recombinant human SphK1 expressed in baculovirus infected Sf9 cells assessed as decrease in [33P]SIP production using D-erythro-sphingo...More data for this Ligand-Target Pair
Affinity DataKi: 3.60nMAssay Description:Inhibition of human C-terminal His6-tagged SphK1 expressed in baculovirus infected Sf21 insect cellsMore data for this Ligand-Target Pair
Affinity DataKi: 3.60nMAssay Description:Inhibition of SphK1 in mouse erythrocytes using sphingosine as substrate by luminescence assayMore data for this Ligand-Target Pair
Affinity DataKi: 3.60nMAssay Description:Inhibition of recombinant human C-terminal His-tagged SPHK1 expressed in fall armyworm sf21 cells using sphingosine as substrate after 1 hr by FITC-b...More data for this Ligand-Target Pair
Affinity DataKi: 3.60nMAssay Description:Inhibition of human recombinant SphK1 expressed in Sf9 cells assessed as radiolabeled products using 5 uM sphingosine and 10 uM gamma[32P]ATP by liqu...More data for this Ligand-Target Pair
Affinity DataKi: 3.60nMAssay Description:Competitive-inhibition of recombinant human C-terminal His6-tagged Sphk1 expressed in baculovirus infected Sf21 insect cells using varying levels of ...More data for this Ligand-Target Pair
Affinity DataKi: 3.60nMAssay Description:Competitive inhibition of recombinant human C-terminal His-tagged SphK1 expressed in baculovirus infected Sf21 cells using varying concentrations of ...More data for this Ligand-Target Pair
Affinity DataKi: 4nMAssay Description:Inhibition of SK1 (unknown origin) using 5 uM of sphingosine as substrateMore data for this Ligand-Target Pair
Affinity DataKi: 4.30nMAssay Description:Competitive inhibition of C-terminal His6-tagged human recombinant SphK1 expressed in baculovirus-infected Sf9 cells using sphingosine as substrateMore data for this Ligand-Target Pair
Affinity DataKi: 4.30nMAssay Description:Inhibition of His6 tagged human SphK1 expressed in Sf21 cells using FITC-sphingosine as substrate preincubated for 90 mins prior substrate addition b...More data for this Ligand-Target Pair
Affinity DataKi: 7.20nMAssay Description:Inhibition of recombinant human Sphk1 expressed in baculovirus infected Sf9 insect cells using d-erythro-sphingosine as substrate measured after 20 m...More data for this Ligand-Target Pair
Affinity DataKi: 8nMAssay Description:Inhibition of recombinant human Sphk1 expressed in baculovirus infected Sf9 insect cells using d-erythro-sphingosine as substrate measured after 20 m...More data for this Ligand-Target Pair
Affinity DataKi: >10nMAssay Description:Inhibition of recombinant human Sphk1 expressed in baculovirus infected Sf9 insect cells using d-erythro-sphingosine as substrate measured after 20 m...More data for this Ligand-Target Pair
Affinity DataKi: >10nMAssay Description:Inhibition of recombinant human Sphk1 expressed in baculovirus infected Sf9 insect cells using d-erythro-sphingosine as substrate measured after 20 m...More data for this Ligand-Target Pair
Affinity DataKi: >10nMAssay Description:Inhibition of recombinant human Sphk1 expressed in baculovirus infected Sf9 insect cells using d-erythro-sphingosine as substrate measured after 20 m...More data for this Ligand-Target Pair
Affinity DataKi: >20nMAssay Description:Inhibition of recombinant human Sphk1 expressed in baculovirus infected Sf9 insect cells using d-erythro-sphingosine as substrate measured after 20 m...More data for this Ligand-Target Pair
Affinity DataKi: 40nMAssay Description:Inhibition of recombinant human SphK1 expressed in baculovirus infected sf9 cells using D-erythro-sphingosine as substrate in presence of [gamma-33P]...More data for this Ligand-Target Pair
Affinity DataKi: 40nMAssay Description:Antagonistic activity against (-)-noradrenaline-induced contraction of rat prostatic vas deferens (Alpha-1A adrenergic receptor )More data for this Ligand-Target Pair
Affinity DataKi: 40nMAssay Description:Inhibition of human SphK1 expressed in baculovirus infected in Sf9 cells using D-erythro-sphingosine as substrate and [gamma-32P]ATP by scintillation...More data for this Ligand-Target Pair
Affinity DataKi: 47nMAssay Description:Inhibition of SK1 (unknown origin)More data for this Ligand-Target Pair
Affinity DataKi: 47nMAssay Description:Inhibition of human recombinant SPHK1 expressed in baculovirus-infected Sf9 cellsMore data for this Ligand-Target Pair
Affinity DataKi: 48nMAssay Description:Inhibition of human recombinant SphK1 expressed in Sf9 cells assessed as radiolabeled products using 5 uM sphingosine and 10 uM gamma[32P]ATP by liqu...More data for this Ligand-Target Pair
Affinity DataKi: 48nMAssay Description:Inhibition of recombinant human SphK1 expressed in baculovirus infected Sf9 cells assessed as decrease in [33P]SIP production using D-erythro-sphingo...More data for this Ligand-Target Pair
Affinity DataKi: 50nMAssay Description:Inhibition of purified human SphK1 assessed as inhibition of formation of [33P]-S1P after 50 mins by scintillation countingMore data for this Ligand-Target Pair
Affinity DataKi: 70nMAssay Description:Inhibition of human SphK1 expressed in baculovirus infected in Sf9 cells using D-erythro-sphingosine as substrate and [gamma-32P]ATP by scintillation...More data for this Ligand-Target Pair
Affinity DataKi: 75nMAssay Description:Inhibition of human recombinant SPHK1 expressed in baculovirus-infected Sf9 cellsMore data for this Ligand-Target Pair
Affinity DataKi: 75nM ΔG°: -42.3kJ/moleT: 2°CAssay Description:Sphingosine kinase activity was assessed as described previously (Kharel, Y., Lee, S., Snyder, A. H., Sheasley-O'Neill, S. L., Morris, M. A., Set...More data for this Ligand-Target Pair
Affinity DataKi: 95nMAssay Description:Inhibition of human recombinant SPHK1 expressed in baculovirus-infected Sf9 cellsMore data for this Ligand-Target Pair
Affinity DataKi: 99nMAssay Description:Inhibition of human recombinant SPHK1 expressed in baculovirus-infected Sf9 cellsMore data for this Ligand-Target Pair
Affinity DataKi: 100nMAssay Description:SLR080811, prepared as the HCl salt, was tested first at recombinant SphK1 and SphK2 using a broken cell assay.More data for this Ligand-Target Pair
Affinity DataKi: 100nM ΔG°: -41.6kJ/moleT: 2°CAssay Description:Sphingosine kinase activity was assessed as described previously (Kharel, Y., Lee, S., Snyder, A. H., Sheasley-O'Neill, S. L., Morris, M. A., Set...More data for this Ligand-Target Pair
Affinity DataKi: 100nMAssay Description:Inhibition of human SphK1 expressed in baculovirus infected in Sf9 cells using D-erythro-sphingosine as substrate and [gamma-32P]ATP by scintillation...More data for this Ligand-Target Pair
Affinity DataKi: 110nM ΔG°: -41.3kJ/moleT: 2°CAssay Description:Sphingosine kinase activity was assessed as described previously (Kharel, Y., Lee, S., Snyder, A. H., Sheasley-O'Neill, S. L., Morris, M. A., Set...More data for this Ligand-Target Pair
Affinity DataKi: 110nMAssay Description:Inhibition of human recombinant SPHK1 expressed in baculovirus-infected Sf9 cellsMore data for this Ligand-Target Pair
Affinity DataKi: 110nMAssay Description:Inhibition of human recombinant SPHK1 expressed in baculovirus-infected Sf9 cellsMore data for this Ligand-Target Pair
Affinity DataKi: 120nMAssay Description:Inhibition of recombinant human SPHK1 using sphingosine as substrate after 30 mins in presence of gamma-[32P]-ATP by liquid scintillation countingMore data for this Ligand-Target Pair
Affinity DataKi: 130nMAssay Description:Inhibition of human recombinant SphK1 expressed in Sf9 cells assessed as radiolabeled products using 5 uM sphingosine and 10 uM gamma[32P]ATP by liqu...More data for this Ligand-Target Pair
Affinity DataKi: 130nMAssay Description:Inhibition of human recombinant SPHK1 expressed in baculovirus-infected Sf9 cellsMore data for this Ligand-Target Pair
Affinity DataKi: 130nM ΔG°: -40.9kJ/moleT: 2°CAssay Description:Sphingosine kinase activity was assessed as described previously (Kharel, Y., Lee, S., Snyder, A. H., Sheasley-O'Neill, S. L., Morris, M. A., Set...More data for this Ligand-Target Pair
Affinity DataKi: 130nMAssay Description:Inhibition of human recombinant SPHK1 expressed in baculovirus-infected Sf9 cellsMore data for this Ligand-Target Pair
Affinity DataKi: 130nMAssay Description:Inhibition of human recombinant SPHK1 expressed in baculovirus-infected Sf9 cellsMore data for this Ligand-Target Pair
Affinity DataKi: 170nM ΔG°: -40.2kJ/moleT: 2°CAssay Description:Sphingosine kinase activity was assessed as described previously (Kharel, Y., Lee, S., Snyder, A. H., Sheasley-O'Neill, S. L., Morris, M. A., Set...More data for this Ligand-Target Pair
Affinity DataKi: 170nMAssay Description:Inhibition of human recombinant SPHK1 expressed in baculovirus-infected Sf9 cellsMore data for this Ligand-Target Pair
Affinity DataKi: 200nMAssay Description:Inhibition of human SphK1 expressed in baculovirus infected in Sf9 cells using D-erythro-sphingosine as substrate and [gamma-32P]ATP by scintillation...More data for this Ligand-Target Pair
Affinity DataKi: 200nM ΔG°: -39.8kJ/moleT: 2°CAssay Description:Sphingosine kinase activity was assessed as described previously (Kharel, Y., Lee, S., Snyder, A. H., Sheasley-O'Neill, S. L., Morris, M. A., Set...More data for this Ligand-Target Pair
Affinity DataKi: 200nMAssay Description:Inhibition of purified human SphK1 assessed as inhibition of formation of [33P]-S1P after 50 mins by scintillation countingMore data for this Ligand-Target Pair
Affinity DataKi: 240nMAssay Description:Inhibition of human recombinant SPHK1 expressed in baculovirus-infected Sf9 cellsMore data for this Ligand-Target Pair
Affinity DataKi: 240nM ΔG°: -39.3kJ/moleT: 2°CAssay Description:Sphingosine kinase activity was assessed as described previously (Kharel, Y., Lee, S., Snyder, A. H., Sheasley-O'Neill, S. L., Morris, M. A., Set...More data for this Ligand-Target Pair
Affinity DataKi: 260nMAssay Description:Inhibition of human SphK1 expressed in baculovirus infected in Sf9 cells using D-erythro-sphingosine as substrate and [gamma-32P]ATP by scintillation...More data for this Ligand-Target Pair
Affinity DataKi: 300nMAssay Description:Antagonistic activity against (-)-noradrenaline-induced contraction of rat thoracic aorta (Alpha-1D adrenergic receptor)More data for this Ligand-Target Pair