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Target
Gag-Pol polyprotein [1148-1435]
Ligand
BDBM27749
Substrate
DIG-tagged Target DNA
Meas. Tech.
DNA Strand-Transfer Activity Assay
pH
7.3±n/a
Temperature
295.15±n/a K
IC50
14±n/a nM
Citation
 Metobo, S; Mish, M; Jin, H; Jabri, S; Lansdown, R; Chen, X; Tsiang, M; Wright, M; Kim, CU Tricyclic HIV integrase inhibitors: VI. SAR studies of 'benzyl flipped' C3-substituted pyrroloquinolines. Bioorg Med Chem Lett 19:1187-90 (2009) [PubMed]  Article 
Target
Name:
Gag-Pol polyprotein [1148-1435]
Synonyms:
HIV-1 Integrase | POL_HV1H2 | gag-pol
Type:
Enzyme
Mol. Mass.:
32171.42
Organism:
Human immunodeficiency virus type 1
Description:
The sequence encoding the 288-amino-acid wild-type full-length integrase was cloned with an N-terminal 6His tag, and expressed in E. coli.
Residue:
288
Sequence:
FLDGIDKAQDEHEKYHSNWRAMASDFNLPPVVAKEIVASCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQETAYFLLKLAGRWPVKTIHTDNGSNFTGATVRAACWWAGIKQEFGIPYNPQSQGVVESMNKELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFRVYYRDSRNPLWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQMAGDDCVASRQDED
  
Inhibitor
Name:
BDBM27749
Synonyms:
3-[(4-fluorophenyl)methyl]-9-hydroxy-5-methoxy-6H,7H,8H-pyrrolo[3,4-g]quinolin-8-one | tricyclic pyrroloquinoline, 9
Type:
Small organic molecule
Emp. Form.:
C19H15FN2O3
Mol. Mass.:
338.3324
SMILES:
COc1c2CNC(=O)c2c(O)c2ncc(Cc3ccc(F)cc3)cc12
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
DIG-tagged Target DNA
Synonyms:
digoxin-tagged DNA
Type:
Duplex DNA
Mol. Mass.:
1978.21
Organism:
n/a
Description:
The biotinylated donor DNA was bound to streptavidin-coated plates.
Residue:
23
Sequence:
TGACCAAGGGCTAATTCACTDIG