TargetBromodomain testis-specific protein [21-137](Homo sapiens (Human))
Dana-Farber Cancer Institute
Dana-Farber Cancer Institute
Affinity DataIC50: 0.574nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [21-137](Homo sapiens (Human))
Dana-Farber Cancer Institute
Dana-Farber Cancer Institute
Affinity DataIC50: 0.878nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [21-137](Homo sapiens (Human))
Dana-Farber Cancer Institute
Dana-Farber Cancer Institute
Affinity DataIC50: 1.09nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [21-137](Homo sapiens (Human))
Dana-Farber Cancer Institute
Dana-Farber Cancer Institute
Affinity DataIC50: 1.59nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
Affinity DataIC50: 1.60nMAssay Description:Inhibition of recombinant human BRDT BD2 (250 to 382 residues) expressed in bacterial expression system by bromoscan assayMore data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [21-137](Homo sapiens (Human))
Dana-Farber Cancer Institute
Dana-Farber Cancer Institute
Affinity DataIC50: 1.84nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [21-137](Homo sapiens (Human))
Dana-Farber Cancer Institute
Dana-Farber Cancer Institute
Affinity DataIC50: 2.36nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
Affinity DataIC50: 3nMAssay Description:Inhibition of BRDT BD2 (unknown origin) using biotinylated JQ1 analog as substrate measured after 20 mins by AlphaLISA assayMore data for this Ligand-Target Pair
Affinity DataIC50: 3nMAssay Description:Inhibition of BRDT BD1 (unknown origin) using biotinylated JQ1 analog as substrate measured after 20 mins by AlphaLISA assayMore data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [21-137](Homo sapiens (Human))
Dana-Farber Cancer Institute
Dana-Farber Cancer Institute
Affinity DataIC50: 5.78nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [21-137](Homo sapiens (Human))
Dana-Farber Cancer Institute
Dana-Farber Cancer Institute
Affinity DataIC50: 6.10nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [21-137](Homo sapiens (Human))
Dana-Farber Cancer Institute
Dana-Farber Cancer Institute
Affinity DataIC50: 6.20nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
Affinity DataIC50: 7.90nMAssay Description:Inhibition of 6His-FLAG-Tev-BRDT (1 to 397 residues) BD2 domain Y309A or Y66A mutant (unknown origin) incubated for 30 mins by TR-FRET assayMore data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [21-137](Homo sapiens (Human))
Dana-Farber Cancer Institute
Dana-Farber Cancer Institute
Affinity DataIC50: 10nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
Affinity DataIC50: 11nMAssay Description:Inhibition of BRDT BD2 (unknown origin) incubated for 30 mins by fluorescence based microtiter plate reader assayMore data for this Ligand-Target Pair
Affinity DataIC50: 11nMAssay Description:Inhibition of BRDT BD1 (unknown origin) incubated for 30 mins by fluorescence based microtiter plate reader assayMore data for this Ligand-Target Pair
Affinity DataIC50: 11nMAssay Description:Inhibition of recombinant BRDT-BD2 (unknown origin) by TR-FRET assayMore data for this Ligand-Target Pair
Affinity DataIC50: 11nMAssay Description:Inhibition of recombinant BRDT-BD1 (unknown origin) by TR-FRET assayMore data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [21-137](Homo sapiens (Human))
Dana-Farber Cancer Institute
Dana-Farber Cancer Institute
Affinity DataIC50: 12.1nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [21-137](Homo sapiens (Human))
Dana-Farber Cancer Institute
Dana-Farber Cancer Institute
Affinity DataIC50: 13.2nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [21-137](Homo sapiens (Human))
Dana-Farber Cancer Institute
Dana-Farber Cancer Institute
Affinity DataIC50: 13.6nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
Affinity DataIC50: 14nMpH: 7.5Assay Description:The assay was carried out in binding mixtures of the bromodomain protein, 0-10 μM compounds and tetra-acetylated histone peptide (SGRGAC.KGGAC.K...More data for this Ligand-Target Pair
Affinity DataIC50: 19nMAssay Description:AlphaScreen is a bead-based, non-radioactive Amplified Luminescent Proximity Homogeneous Assay. The details are not described in this patent.More data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [21-137](Homo sapiens (Human))
Dana-Farber Cancer Institute
Dana-Farber Cancer Institute
Affinity DataIC50: 21.7nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
Affinity DataIC50: 22.5nMAssay Description:AlphaScreen is a bead-based, non-radioactive Amplified Luminescent Proximity Homogeneous Assay. The details are not described in this patent.More data for this Ligand-Target Pair
Affinity DataIC50: 23nMpH: 7.5Assay Description:The assay was carried out in binding mixtures of the bromodomain protein, 0-10 μM compounds and tetra-acetylated histone peptide (SGRGAC.KGGAC.K...More data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [21-137](Homo sapiens (Human))
Dana-Farber Cancer Institute
Dana-Farber Cancer Institute
Affinity DataIC50: 23.2nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
Affinity DataIC50: 25nMAssay Description:Displacement of Alexa Fluor 647 labelled I-BET762 from N-terminal His6-tagged human BRDT BD1 (1 to 397 residues)/BD2 Y309A mutant expressed in Escher...More data for this Ligand-Target Pair
Affinity DataIC50: 25nMAssay Description:Binding affinity to 6His-FLAG-Tev-BRDT (1 to 397 residues) BD2 domain Y309A or Y66A mutant (unknown origin) incubated for 30 mins by TR-FRET assayMore data for this Ligand-Target Pair
Affinity DataIC50: 25nMAssay Description:Displacement of Alexa Fluor 647 labelled ligand from 6His-Thr tagged BRDT BD2/BD1 Y66A mutant (1 to 397 residues) (unknown origin) measured after 30 ...More data for this Ligand-Target Pair
Affinity DataIC50: 27.8nMAssay Description:AlphaScreen is a bead-based, non-radioactive Amplified Luminescent Proximity Homogeneous Assay. The details are not described in this patent.More data for this Ligand-Target Pair
Affinity DataIC50: 29.1nMAssay Description:AlphaScreen is a bead-based, non-radioactive Amplified Luminescent Proximity Homogeneous Assay. The details are not described in this patent.More data for this Ligand-Target Pair
Affinity DataIC50: 31nMAssay Description:The bromodomain binding assays were performed by Reaction Biology Corp., Malvern, Pa., USA (www.reactionbiology.com). The BET binding assays were con...More data for this Ligand-Target Pair
Affinity DataIC50: 31nMpH: 7.5Assay Description:The assay was carried out in binding mixtures of the bromodomain protein, 0-10 μM compounds and tetra-acetylated histone peptide (SGRGAC.KGGAC.K...More data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [21-137](Homo sapiens (Human))
Dana-Farber Cancer Institute
Dana-Farber Cancer Institute
Affinity DataIC50: 31.7nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
Affinity DataIC50: 35nMAssay Description:Inhibition of BRDT (unknown origin)More data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [21-137](Homo sapiens (Human))
Dana-Farber Cancer Institute
Dana-Farber Cancer Institute
Affinity DataIC50: 40.3nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
Affinity DataIC50: 41.3nMAssay Description:AlphaScreen is a bead-based, non-radioactive Amplified Luminescent Proximity Homogeneous Assay. The details are not described in this patent.More data for this Ligand-Target Pair
Affinity DataIC50: 42nMpH: 7.5Assay Description:The assay was carried out in binding mixtures of the bromodomain protein, 0-10 μM compounds and tetra-acetylated histone peptide (SGRGAC.KGGAC.K...More data for this Ligand-Target Pair
Affinity DataIC50: 46nMAssay Description:Homogeneous time=resolved fluorescence (HTRF) was used to detect the binding of the compound to BRD4(D1 + D2) and BRDT (D1) proteins, and the AlphaSc...More data for this Ligand-Target Pair
Affinity DataIC50: 46nMAssay Description:Homogeneous time=resolved fluorescence (HTRF) was used to detect the binding of the compound to BRD4(D1 + D2) and BRDT (D1) proteins, and the AlphaSc...More data for this Ligand-Target Pair
Affinity DataIC50: 49nMAssay Description:Homogeneous time=resolved fluorescence (HTRF) was used to detect the binding of the compound to BRD4(D1 + D2) and BRDT (D1) proteins, and the AlphaSc...More data for this Ligand-Target Pair
Affinity DataIC50: 50nMAssay Description:Inhibition of 6His-FLAG-Tev-BRDT (1 to 397 residues) BD2 domain Y309A or Y66A mutant (unknown origin) incubated for 30 mins by TR-FRET assayMore data for this Ligand-Target Pair
Affinity DataIC50: 50nMAssay Description:Inhibition of BRDT (unknown origin) by TR-FRET assayMore data for this Ligand-Target Pair
Affinity DataIC50: 51nMAssay Description:Homogeneous time=resolved fluorescence (HTRF) was used to detect the binding of the compound to BRD4(D1 + D2) and BRDT (D1) proteins, and the AlphaSc...More data for this Ligand-Target Pair
Affinity DataIC50: 58nMpH: 7.5Assay Description:The assay was carried out in binding mixtures of the bromodomain protein, 0-10 μM compounds and tetra-acetylated histone peptide (SGRGAC.KGGAC.K...More data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [22-138](Homo sapiens (Human))
Dana-Farber Cancer Institute
US Patent
Dana-Farber Cancer Institute
US Patent
Affinity DataIC50: 60nMAssay Description:Acetyl-Histone Binding Assay. Assays were performed as described previously with minor modifications from the manufacturer's protocol (PerkinElme...More data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [21-137](Homo sapiens (Human))
Dana-Farber Cancer Institute
Dana-Farber Cancer Institute
Affinity DataIC50: 60.2nMpH: 7.5 T: 2°CAssay Description:Assays were performed with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were diluted in 50 mM HEPES, 150...More data for this Ligand-Target Pair
TargetBromodomain testis-specific protein [22-138](Homo sapiens (Human))
Dana-Farber Cancer Institute
US Patent
Dana-Farber Cancer Institute
US Patent
Affinity DataIC50: 61nMAssay Description:Assays were performed as described previously with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were dil...More data for this Ligand-Target Pair
Affinity DataIC50: 61nMAssay Description:ssays were performed as described previously with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were dilu...More data for this Ligand-Target Pair
